大豆生长节间响应温度和外源GA诱导的赤霉素途径关键基因分析  

Analysis of key genes involved in GA pathway responding to temperature and exogenous GA related to internode development in soybean

作　　者：齐阳阳 窦汝娜 赵彩桐 张帜 李文滨[1] 姜振峰[1] QI Yang-Yang;DOU Ru-Na;ZHAO Cai-Tong;ZHANG Zhi;LI Wen-Bin;JIANG Zhen-Feng(Northeast Agricultural University,Key Laboratory of Soybean Biology in Chinese Ministry of Education/Key Laboratory of Soybean Biology and Genetics Breeding of Ministry of Agriculture and Rural Affairs,Harbin 150030,Heilongjiang,China)

机构地区：[1]东北农业大学大豆生物学教育部重点实验室/农业农村部东北大豆生物学与遗传育种重点实验室,黑龙江哈尔滨150030

出　　处：《作物学报》2023年第1期62-72,共11页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31571693,32172072);财政部和农业农村部国家现代农业产业技术体系建设专项(大豆,重点任务)(CARS-04-04B);黑龙江省自然科学基金项目(LH2021C025)资助。

摘　　要：为了解大豆节间在不同温度和外源赤霉素(gibberellic acid,GA)诱导条件下的表型变化规律,分析GA合成重要途径和挖掘调控节间的关键候选基因,本研究将大豆品种Charleston在培养箱条件和室外盆栽条件种植,进行不同温度处理和外源GA涂抹处理,利用徒手切片配合显微照相方法,分析大豆节间长度和细胞形态变化;利用液相色谱-质谱联机结合转录组测序方法分析大豆节间GA合成主要通路并挖掘调控节间生长的关键候选基因。结果表明在25℃和30℃条件下,外源涂抹不同浓度GA可以诱导大豆生长节间伸长,随着伸长量的增加,大豆节间都变得纤细。外源GA对细胞作用效果主要为促进伸长,对宽度影响不明显。高温处理对节间的伸长效果高于低温处理。本研究鉴定到GA2氧化酶基因在大豆生长节间表达和较高含量的GA19和GA53,及这2种GA下游的GA20(活性GA前体),以及这条合成途径的活性GA产物GA3也被检测到都存在于细胞伸长区组织,说明从GA前体物质到GA53,再到GA19,通过GA20最终合成GA3是大豆节间生长的一条重要GA合成通路,进一步说明GA2氧化酶在大豆节间生长过程中有重要作用。挖掘到某些DELLA、GA和PIF基因家族成员具有组织表达特异性,为调节大豆节间和株高提供了候选基因。The objective of this study is to explore the effects of temperature and exogenous GA on the development of soybean internode and the key genes related to gibberellin(GA)signal transduction pathway.The soybean variety‘Charleston’was grown in chambers or in pots under outdoor condition,and subjected to the treatments with different temperature and concentration of GA solutions.Phenotype observation,section with hand,LC-MS analysis,and RNA-seq experiments were performed to investigate the internode changes and the genes involved in the GA signal transduction pathway.Different temperature and exogenous GA all induced the internode elongation and the longer the length increased,the slender the internode became.Exogenous GA had an evident elongating effect on the cell length of the internode despite of no effect on the cell width.The internodes grown in 30℃growth condition were longer than those in 25℃growth condition.GA2-oxidase,GA19,GA53,GA20,and bioactive GA3 were detected in the elongation zone of soybean internode,suggesting that GA2-oxidase might play an important role in the developmental process of soybean internode.Tissue-specific genes in DELLA,GAI,and PIF gene families could be identified and facilitate the gene selection to regulate the internode growth and plant height from GA signal transduction pathway.Soybean internode was regulated by temperature and exogenous GA.From GA53 to GA19 to GA20 and ultimately to GA3 was an important GA synthesis pathway.GA2-oxidase played an important role on the internode development of soybean.The GA-GID1-DELLA complex was expressed specifically in soybean internode.The candidate genes related to the internode development could be filtered from GID1 and DELLA gene family.

关 键 词：大豆 生长节间 GA途径 GA-GID1-DELLA 

分 类 号：S565.1[农业科学—作物学]