基于网络药理学研究栝楼-薤白药对治疗冠状动脉粥样硬化性心脏病作用机制  

A study on the action mechanism of Gualou-Xiebai medicine pair on coronary atherosclerotic heart disease based on network pharmacology

作　　者：马月 姜钧文[2] MA Yue

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中医临床研究》2022年第28期12-18,共7页Clinical Journal Of Chinese Medicine

基　　金：辽宁省自然科学基金:基于Pink1-Mfn2-Parkin通路探讨健脾祛痰方调控动脉粥样硬化的作用机制研究(2019-ZD-0438)。

摘　　要：目的:基于网络药理学探讨栝楼-薤白药对治疗冠状动脉粥样硬化性心脏病(简称冠心病)的作用机制。方法:检索中药系统药理学数据库与分析平台(TCMSP)数据库,用PubChem查询各成分的SMILE结构,通过Swiss Target Prediction和DrugBank进行靶点预测,通过GeneCards、DisGeNET、TTD检索与冠心病相关的靶点基因,均导入UniProt进行基因标准化,再用Venny 2.1.0获取预测靶点的交集。利用STRING数据库、Cytoscape 3.9.0构建中药-成分-靶点网络。将最终作用靶点基因录入Metascape网站进行基因本体-分子功能、基因本体-细胞成分、基因本体-生物过程及京都基因与基因组百科全书(KEGG)分析,将数据导入OmicShare网站绘制气泡图,构建靶点-通路网络模型。结果:共筛选出具有较高活性的化学成分22个,度值较高的是香叶木素、亚油酸乙酯、α-菠菜甾醇、柚皮素、前列腺素A1,筛选出核心靶点为酪氨酸激酶、类视黄醇X受体、雌激素受体1、信号传导转录激活因子3、丝裂原活化蛋白激酶。筛选出与冠心病相关的化学致癌-受体激活通路等核心通路。结论:香叶木素、亚油酸乙酯、α-菠菜甾醇、柚皮素、前列腺素等可能是栝楼-薤白药对治疗冠心病发挥关键作用的活性物质,可能通过作用于酪氨酸激酶、类视黄醇X受体、雌激素受体1、信号传导转录激活因子3、丝裂原活化蛋白激酶等靶点对化学致癌-受体激活通路等通路进行调控。栝楼-薤白药对可能通过抗炎、调节脂代谢、稳定斑块、血管保护等作用治疗冠心病。Objective:To explore the action mechanism of Gualou-Xiebai medicine pair in the treatment of coronary atherosclerotic heart disease based on network pharmacology.Methods:tcmsp database was searched,and PubChem was used to query the smile structure of each component.Swisstargetprediction and DrugBan were used to predict the target.The target genes related to coronary atherosclerotic heart disease(CHD)were searched by GeneCards,DisGeNE and therapeutic target database(TTD).All of them were imported into UniProt for gene standardization,and then Venny 2.1.0 was used to take the intersection of prediction targets.String database and Cytoscape 3.9.0 was used to construct traditional Chinese medicine-component-target network.The final target genes were imported into metascape website for go-mf,go-cc,go-bp and KEGG analysis.The data was imported into OmicShare website to draw a bubble chart and build a targetpathway network model.Results:A total of 22 chemical constituents with higher activity were screened out.The ones with higher degree values were geranialignin,ethyl linoleate,α-spinasterol,naringenin,prostaglandin A1.The screened core targets were tyrosine kinase,retinoid X receptor,estrogen receptor 1,signal transduction transcription activator 3,and mitogen-activated protein kinase.Core pathways such as chemical oncogenic-receptor activation pathways related to coronary heart disease were screened.Conclusions:Geranilin,ethyl linoleate,α-spinachsterol,naringenin,prostaglandin,etc.may be the active substances of Gualou-Xiebai medicine pair that play a key role in the treatment of coronary atherosclerotic heart disease.The Gualou-Xiebai medicine pair may affect cchemical oncogenic-receptor activation pathway and so on by acting on targets such as tyrosine kinases,retinoid X receptors,estrogen receptor 1,signaling activator of transcription 3,and mitogen-activated protein kinase.Gualou-Xiebai medicine pair may treat coronary atherosclerotic heart disease by antiinflammatory,regulating lipid metabolism,stabilizing pla

关 键 词：冠状动脉粥样硬化性心脏病 栝楼-薤白药对 网络药理学 

分 类 号：R256.2[医药卫生—中医内科学]