L_(9)(3^(4))正交试验法优选黄芩解毒颗粒水提取工艺  

Optimization of Water Extraction Process of Huangqin Jiedu Granules by the L_(9)(3^(4))Orthogonal Test

作　　者：罗朝亮[1] 韦开听[1] 龙立活[1] 陆治宇 周贤强 张浩 吴芳芳 陈路[1] LUO Chaoliang;WEI Kaiting;LONG Lihuo;LU Zhiyu;ZHOU Xianqiang;ZHANG Hao;WU Fangfang;CHEN Lu(Guangxi Botanical Garden of Madicinal Plants·National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Nanning,Guangxi,China 530023;Guangxi Zhuangyaofang Hospital,Nanning,Guangxi,China 530150)

机构地区：[1]广西壮族自治区药用植物园·西南濒危药材资源开发国家工程实验室,广西南宁530023 [2]广西壮要方医院,广西南宁530150

出　　处：《中国药业》2022年第21期44-48,共5页China Pharmaceuticals

基　　金：广西壮族自治区科学技术厅科技项目[桂科ZY21195044,桂科AD18126013];广西壮族自治区南宁市科学研究与技术开发计划项目[20203109];广西壮族自治区药用植物园创新团队计划项目[桂药创2019001]。

摘　　要：目的优选黄芩解毒颗粒的水提取工艺。方法以加水量、煎煮时间和煎煮次数为考察因素,以干膏得率和主药黄芩中指标性成分黄芩苷的回收率为评价指标,采用L_(9)(3^(4))正交试验优选提取工艺,并进行验证试验。结果最佳提取工艺为加8倍量水,煎煮2次,每次1.0 h。所制备样品的平均干膏得率和黄芩苷回收率分别为27.51%和44.65%,RSD分别为0.93%和0.61%(n=3)。结论优选的提取工艺操作简便、工艺稳定,可用于黄芩解毒颗粒的工业化生产。Objective To optimize the water extraction process of Huangqin Jiedu Granules.Methods The extraction process was optimized by the L_(9)(3^(4))orthogonal test with the amount of water addition,decocting time and decocting times as the investigaion factors,and with the yield of dry extract,recovery rate of baicalin,the index component in the main drug of Scutellariae Radix,as the evaluation indexes.Then the verification test was carried out.Results The optimal extraction process was as follows:adding eight times the amount of water,decocting twice,each time for 1.0 h.The average yield of dry extract and the average recovery rate of baicalin in the prepared samples were 27.51%and 44.65%with RSDs of 0.93%and 0.61%(n=3)respectively.Conclusion The optimal extraction process is simple and stable,which can be used for the industrial production of Huangqin Jiedu Granules.

关 键 词：黄芩解毒颗粒 L_(9)(3^(4))正交试验 水提取法 工艺优选 

分 类 号：R932[医药卫生—生药学] TQ460.6[医药卫生—药学]