高效液相色谱波长切换法同时测定黄疸茵陈颗粒中8种指标性成分的含量  

Simultaneous Determination of Eight Index Components in Huangdan Yinchen Granules by HPLC with Wavelength Switching

作　　者：毛淑倩 MAO Shuqian(The 900th Hospital of the Joint Logistics Support Force of Chinese PLA,Fuzhou,Fujian,China 350000)

机构地区：[1]中国人民解放军联勤保障部队第九○○医院,福建福州350000

出　　处：《中国药业》2022年第21期69-72,共4页China Pharmaceuticals

摘　　要：目的建立同时测定黄疸茵陈颗粒中绿原酸、滨蒿内酯、黄芩苷、汉黄芩素、黄芩素、汉黄芩苷、大黄酚、甘草酸铵8种指标性成分含量的高效液相色谱(HPLC)波长切换法。方法色谱柱为Supfex JX C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.12%甲酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为0~44 min时325 nm(绿原酸、滨蒿内酯、黄芩苷、汉黄芩素、黄芩素、汉黄芩苷)、44~50 min时237 nm(甘草酸铵)、50~60 min时258 nm(大黄酚),柱温为35℃,进样量为10μL。结果绿原酸、滨蒿内酯、黄芩苷、汉黄芩素、黄芩素、汉黄芩苷、大黄酚、甘草酸铵的质量浓度分别在12.080~120.80μg/mL(r=0.9998)、2.986~29.86μg/mL(r=0.9995)、9.753~97.53μg/mL(r=0.9997)、3.611~36.11μg/mL(r=0.9993)、6.114~61.14μg/mL(r=0.9994)、3.707~37.07μg/mL(r=0.9991)、5.665~56.65μg/mL(r=0.9996)、5.819~58.19μg/mL(r=0.9995)范围内与峰面积线性关系良好;精密度、稳定性、重复性试验结果的RSD均小于1.5%(n=6));平均加样回收率分别为100.11%,98.81%,99.37%,98.52%,98.83%,98.55%,99.72%,99.56%,RSD分别为1.26%,0.44%,0.63%,0.93%,1.01%,1.11%,0.91%,0.72%(n=6)。结论该方法操作简便、准确度高、重复性好,可用于同时测定黄疸茵陈颗粒中8种指标性成分的含量。Objective To establish a high-performance liquid chromatography(HPLC)with wavelength switching method for the simultaneous determination of eight index components in Huangdan Yinchen Granules,including clorogenic acid,scoparone,baicalin,wogonin,baicalein,wogonoside,chrysophanol and ammonium glycyrrhizinate.Methods The chromatographic column was Supfex JX C_(18) column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.12%formic acid solution(gradient elution),the flow rate was 1.0 mL/min.The detection wavelengths were 325 nm for clorogenic acid,scoparone,baicalin,wogonin,baicalein and wogonoside at 0-44 min,237 nm for ammonium glycyrrhizinate at 44-50 min,258 nm for chrysophanol at 50-60 min.The column temperature was 35℃,and the injection volume was 10μL.Results The linear ranges of clorogenic acid,scoparone,baicalin,wogonin,baicalein,wogonoside,chrysophanol and ammonium glycyrrhizinate were 12.080-120.80μg/mL(r=0.9998),2.986-29.86μg/mL(r=0.9995),9.753-97.53μg/mL(r=0.9997),3.611-36.11μg/mL(r=0.9993),6.114-61.14μg/mL(r=0.9994),3.707-37.07μg/mL(r=0.9991),5.665-56.65μg/mL(r=0.9996)and 5.819-58.19μg/mL(r=0.9995),respectively.The RSDs of precision,stability and repeatability tests were lower than 1.5%(n=6).The average recoveries of the above index components were 100.11%,98.81%,99.37%,98.52%,98.83%,98.55%,99.72%and 99.56%with RSDs of 1.26%,0.44%,0.63%,0.93%,1.01%,1.11%,0.91%and 0.72%respectively(n=6).Conclusion The method is simple,accurate and repeatable,which can be used for the simultaneous determination of eight index components in Huangdan Yinchen Granules.

关 键 词：黄疸茵陈颗粒 高效液相色谱法 波长切换法 指标性成分 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0