Cytological study on haploid male fertility in maize  

作　　者：YANG Ji-wei LIU Zong-hua QU Yan-zhi ZHANG Ya-zhou LI Hao-chuan 

机构地区：[1]College of Agronomy,Henan Agricultural University/State Key Laboratory of Wheat and Maize Crop Science/Henan Grain Crop Collaborative Innovation Center,Zhengzhou 450046,P.R.China [2]Institute of Cereal Crops,Henan Academy of Agricultural Sciences,Zhengzhou 450002,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第11期3158-3168,共11页农业科学学报（英文版）

基　　金：supported by the Agricultural Seed Joint Research Project of Henan Province,China(2022010202);the Science and Technology Project of Henan Province,China(222102110276);the China Postdoctoral Science Foundation(2020M682031)。

摘　　要：Doubled haploid(DH)breeding technology,which relies on haploid genome doubling,is widely used in commercial maize breeding.Spontaneous haploid genome doubling(SHGD),a more simplified and straightforward method,is gaining popularity among maize breeders.However,the cytological mechanism of SHGD remains unclear.This study crossed inbred lines RL36 and RL7,which have differing SHGD abilities,with inducer line YHI-1 to obtain haploid kernels.The meiotic processes of pollen mother cells(PMCs)in the haploid plants were compared with diploid controls.The results suggested that three main pathways,the early doubling of haploid PMCs,the first meiotic metaphase chromosomal segregation distortion,and anomaly of the second meiosis,are responsible for SHGD.Furthermore,flow cytometry analysis of ploidy levels in leaves and PMCs from haploids and diploid controls revealed that somatic cell chromosome doubling and germ cell chromosome doubling are independent processes.These findings provide a foundation for further studies on the underlying mechanism of SHGD,aiding the application of DH technology in maize breeding practices.

关 键 词：MAIZE HAPLOID male fertility spontaneous genome doubling MEIOSIS 

分 类 号：S513[农业科学—作物学]