一种Fe^(3+)荧光增强型探针的设计、合成及应用  

Design, Synthesis and Application of a Fe^(3+) Luorescent Enhanced Probe

作　　者：黄磊 包磊 郑蕾 郭景春 李超 胡雨阳 赵溪 鲁敏 HUANG Lei;BAO Lei;ZHENG Lei;GUO Jingchun;LI Chao;HU Yuyang;ZHAO Xi;LU Min(Department of experimental and practical teaching management,West Anhui University,Lu’an Anhui 237012,China)

机构地区：[1]皖西学院实验实训教学管理部,安徽六安237012

出　　处：《阜阳师范大学学报（自然科学版）》2022年第3期37-42,共6页Journal of Fuyang Normal University:Natural Science

基　　金：安徽省高校科学研究项目(KJ2020A0629);皖西学院科研自然重点项目(WXZR202218);大学生创新创业项目(S202110376038)资助。

摘　　要：设计合成了三芳基咪唑衍生物探针P,利用^(1)H NMR、^(13)C NMR、HRMS等表征手段确认其结构。荧光光谱测试研究表明在PBS-THF溶液(v/v,1:9,pH=7)中,Fe^(3+)的加入会引起探针P溶液荧光增强,并且探针P对Fe^(3+)具有选择性、抗干扰性、灵敏性等良好的性能。在0~10µmol/L范围内,499 nm处的荧光强度差值(F-F0)与Fe^(3+)浓度呈良好的线性关系(R^(2)=0.99646),检测限为0.5µmol/L。Job’s曲线、EDTA可逆性实验、1 H NMR滴定、HRMS实验验证了探针P与Fe^(3+)之间配位作用机理。探针P可以固载于试纸条上检验溶液中的Fe^(3+),还可以测定实际水样中的Fe^(3+)含量并获得94.7%~106.0%回收率。Triarylimidazole derivatives based probe P was designed and synthesized and its structure was confirmed by 1 H NMR,13C NMR and HRMS.Fluorescence spectrum test showed that probe P solution(PBS-THF,v/v,1:9,pH=7)could enhance with the addition of Fe^(3+)and had good performance of selectivity,anti-interference and sensitivity to Fe^(3+).In the range of 0~10µmol/L,the fluorescence intensity difference value at 499 nm(F-F0)showed a good linear relationship with the concentration of Fe^(3+)(R^(2)=0.99646)and the detection limit was 0.5µmol/L.The coordination mechanism between probe P and Fe^(3+)was verified by Job's curve,EDTA reversibility experiment,1 H NMR titration experiment and HRMS.The probe P can be fixed on the paper strip for the determination of Fe^(3+)in solution and can be also used to determine the content of Fe^(3+)in actual water samples with 94.7%~106.0%recoveries.

关 键 词：三芳基咪唑衍生物 Fe^(3+) 荧光探针 试纸条 

分 类 号：O657.3[理学—分析化学]