振动和水流对拉萨裸裂尻鱼受精卵孵化性能的影响  

Effects of Vibration and Water Low on Hatching Performance of Schizopygopsis Younghusbandi Fertilized Eggs

作　　者：曾本和 方媛林 何文佳 王金林 ZENG Benhe;FANG Yuanlin;HE Wenjia;WANG Jinlin(Institute of Fisheries Science,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,China;School of fisheries,Southwest University,Chongqing 404100,China)

机构地区：[1]西藏自治区农牧科学院水产科学研究所,西藏拉萨850000 [2]西南大学水产学院,重庆404100

出　　处：《高原科学研究》2022年第3期37-44,共8页Plateau Science Research

基　　金：西藏自治区外国专家项目(2021WZ001);西藏自治区自然科学基金项目(XZ2021012R0064G).

摘　　要：为探究水流对拉萨裸裂尻鱼孵化性能的影响,以同一批繁殖获得受精卵为研究对象,开展连续震荡和间歇性震荡对孵化率影响实验,确定拉萨裸裂尻鱼受精卵对振动和水流的敏感性,再通过瓶式孵化器及孵化盘孵化拉萨裸裂尻鱼受精卵,以验证试验结果。结果表明:(1)连续振动下对照组和100 r/min组死亡率均较低,且差异不显著(P>0.05);振动频率提高到150 r/min时,拉萨裸裂尻鱼受精卵死亡率显著升高(P<0.05),死卵主要集中在原肠早期至肌节出现期。(2)间歇性振动下,对照组各发育时期死亡率均较低,50 r/min组原肠中期死亡率最高,100 r/min组神经胚期死亡率最高,150 r/min组胚孔封闭期死亡率最高,200 r/min组原肠中期死亡率最高,250 r/min组原肠早期和出膜期死亡率最高。(3)在流速为(0.314±0.025)m/s瓶式孵化器及流速为(0.013±0.001)m/s孵化盘中拉萨裸裂尻鱼受精卵孵化率和畸形率均差异不显著(P>0.05)。综上所述,拉萨裸裂尻鱼受精卵对振动和水流的敏感期均集中在原肠早期至肌节出现期,其规模化孵化可在瓶式孵化器中孵化,肌节出现期以前水流速应尽量调小,水流速低于0.314 m/s,肌节出现期以后可适量调大。In order to explore the influence of water flow on the hatching performance of Schizopygopsis younghusbandi.Taking the fertilized eggs obtained from the same batch of reproduction as the research object.Carry out experiments on the influence of continuous and intermittent shocks on hatching rate.Determine the sensitivity of Schizopygopsis younghusbandi fertilized eggs to vibration and water flow.Then the Schizopygopsis younghus⁃bandi fertilized eggs were incubated in a bottle incubator and an incubator to verify the test results.The results showed that:(1)The mortality of the control group and the 100 r/min group under continuous vibration was lower,and the difference was not significant(P>0.05),When the vibration frequency was increased to 150 r/min,the mortality of fertilized eggs of Schizopygopsis younghusbandi increased significantly(P<0.05).The dead eggs were mainly concentrated in the early stage of gastrula to the emergence of sarcomere.(2)Under intermittent vi⁃bration,the mortality of the control group at all developmental stages was low.The mortality of the 50 r/min group was the highest at the mid gastrula stage,the 100 r/min group at the neuroembryonic stage,the 150 r/min group at the blastopore closure stage,the 200 r/min group at the mid gastrula stage,and the 250 r/min group at the early gastrula stage and at the membranous stage were the highest.(3)There was no significant difference in hatching rate and deformity rate of fertilized eggs between(0.314±0.025)m/s bottle incubator and(0.013±0.001)m/s incubator(P>0.05).So,the sensitive period of the Schizopygopsis younghusbandi fertilized eggs to vibration and water flow is concentrated in the early gastrula to the onset of sarcomere.Their large-scale hatching can be carried out in a bottle incubator.Before the onset of sarcomere,the water flow velocity should be as low as possi⁃ble.The water flow velocity should be lower than 0.314 m/s.After the onset of sarcomere,it can be increased ap⁃propriately.

关 键 词：拉萨裸裂尻鱼 受精卵 发育时期 敏感期 孵化方法 优化 

分 类 号：S931.1[农业科学—渔业资源]