Effects of Different Concentrations of Radix pseudostellariae Fibrous Root Extracts on Serum Cytokines in Mice and Their Correlation Analysis  

不同浓度太子参须提取物对小鼠血清细胞因子的影响及其相关性分析

作　　者：PAN Hui-qing ZHANG Yan-da YI Wei-meng CHEN Sai-hong JIA Zhi-ping ZHAO Qi MA Yu-fang 潘慧青;张炎达;衣伟萌;陈赛红;贾志平;赵齐;马玉芳(福建贝迪药业有限公司,福建宁德355300;中西兽医结合与动物保健福建省高等学校重点实验室,福建福州350002)

机构地区：[1]Fujian Beidi Pharmaceutical Co.,Ltd.,Ningde 355300,PRC [2]Key Laboratory of Colleges for the Combination of Chinese and Western Veterinary Medicine and Animal Health in Fujian Province,Fuzhou 350002,PRC

出　　处：《Agricultural Science & Technology》2022年第3期46-51,共6页农业科学与技术（英文版）

基　　金：中央引导地方科技发展专项(2021L3030);福建农林大学校企合作横向课题(104/KH180151A)。

摘　　要：To investigate the effects of Radix pseudostellariae fibrous root extracts(RPFRE) on serum cytokines in normal and immunosuppressed mice and the correlation between indexes, KM male mice were randomly divided into four groups, which were given 0(control), 0.1, 0.2, 0.4 g/kg RPFRE by gavage. The levels of IL-2, IL-4, IL-6, and IFN-γ in the serum of mice were measured 14 d after gavage. On the 15day, the remaining mice in the experimental group were intraperitoneally injected with cyclophosphamide(CY), and samples were collected on the 18day to measure serum cytokines levels. The results showed that RPFRE improved the serum cytokine level in normal mice. 0.1 g/kg RPFRE significantly increased the content of IL-2and IFN-γ(P<0.05), while 0.4 g/kg RPFRE significantly increased the content of IL-4 and IL-6(P<0.05). Correlation analysis showed that serum cytokines IL-4 and IL-6 in normal mice had an extremely significant positive correlation with RPFRE concentration(P<0.01), IL-2 had a significant or extremely significant positive correlation with IL-4, IL-6, and IFN-γ(P<0.01or P<0.05), and IL-4 had an extremely significant positive correlation with IL-6(P<0.01). After CY injection, serum cytokine levels in mice decreased and immune function was inhibited. Compared with the control group, the experimental group recovered the level of cytokines to some extent. 0.1 g/kg RPFRE significantly increased the content of IL-2 in immunosuppressed mice(P<0.05), and 0.4 g/kg RPFRE significantly increased the content of IL-4, IL-6, and IFN-γ in immunosuppressed mice(P<0.05). Correlation analysis showed that the serum cytokines in immunosuppressed mice were significantly positively correlated with RPFRE concentration(P<0.01), and there was a certain dose dependence. Moreover, the correlation coefficient between IL-2 and IL-4, IL-6, and IFN-γ, IL-4 and IL-6, and IL-6 and IFN-γ reached an extremely significant level(P<0.01). To sum up, RPFRE could improve the serum cytokines level of mice, promote the secretion of cytokines, and 为探究太子参须提取物(RPFRE)对正常与免疫抑制小鼠血清细胞因子的影响及其指标间相关性关系,选用KM雄性小鼠随机分为四组,分别灌胃0(对照)、0.1、0.2、0.4 g/kg RPFRE。14天后分别测定小鼠血清细胞因子IL-2、IL-4、IL-6、IFN-γ含量;第15~17天,实验组剩余小鼠均腹腔注射环磷酰胺(CY),第18天采样测定上述指标。结果显示,RPFRE灌胃14天后,正常小鼠血清细胞因子水平明显提高。0.1 g/kg RPFRE可显著提升IL-2、IFN-γ含量(P <0.05),0.4 g/kg RPFRE可显著提升IL-4、IL-6含量(P <0.05)。相关性分析表明RPFRE用药浓度与正常小鼠血清细胞因子IL-4、IL-6具有极显著正向相关关系(P <0.01),IL-2与IL-4、IL-6和IFN-γ具有显著或极显著正向相关关系(P <0.01或P <0.05),IL-4与IL-6具有极显著正向相关关系(P<0.01)。注射CY后,小鼠血清细胞因子水平降低,免疫功能受到抑制。与对照相比,实验组的细胞因子水平有所恢复,0.1 g/kg RPFRE显著提高免疫抑制小鼠IL-2含量(P <0.05),0.4 g/kg RPFRE显著提高免疫抑制小鼠IL-4、IL-6、IFN-γ含量(P <0.05)。相关性分析结果显示RPFRE用药浓度与免疫抑制小鼠血清细胞因子具有极显著正向相关关系(P <0.01),且存在一定的剂量依赖性,IL-2与IL-4、IL-6和IFN-γ之间,IL-4与IL-6之间,IL-6与IFN-γ之间均具有极显著正向相关关系(P <0.01)。综上所述,RPFRE可提升小鼠的血清细胞因子水平,促进细胞因子分泌,具有一定免疫保护作用,且RPFRE用药浓度与小鼠血清细胞因子间具有显著相关性,为RPFRE免疫功能的深入研究提供了基础数据支撑。

关 键 词：Radix pseudostellariae fibrous root Extraction MICE CYTOKINES CORRELATION 

分 类 号：R285.5[医药卫生—中药学]