双相磷酸钙涂覆羟基磷灰石多孔支架的制备及性能  

Preparation and properties of porous hydroxyapatite scaffolds with biphase calcium phosphate coating

作　　者：李京娜 曾庆丰 于树印[3] 秦月 艾子政 董谢平 Li Jingna;Zeng Qingfeng;Yu Shuyin;Qin Yue;Ai Zizheng;Dong Xieping(Department of Orthopedics,Jiangxi Provincial People’s Hospital,Nanchang 330006,Jiangxi Province,China;Graduate School of Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China;MSEA International Institute for Materials Genome,Langfang 065500,Hebei Province,China;School of Materials Science and Engineering,Northwestern Polytechnical University,Xi’an 710072,Shaanxi Province,China;The Fourth Hospital of Nanchang,Nanchang 330104,Jiangxi Province,China;Medical College of Nanchang University,Nanchang 330006,Jiangxi Province,China;Digital Lab of Orthopeadics,Key Laboratory of Health Commission of Jiangxi Province,Nanchang 330006,Jiangxi Province,China;Intelligent and Digital Surgery Innovation Center of SUSTech,Southern University of Science and Technology Hospital,Shenzhen 518000,Guangdong Province,China)

机构地区：[1]江西省人民医院骨科,江西省南昌市330006 [2]江西中医药大学研究生院,江西省南昌市330004 [3]迈海材料基因组国际研究院,河北省廊坊市065500 [4]西北工业大学材料学院,陕西省西安市710072 [5]南昌市第四医院,江西省南昌市330104 [6]南昌大学医学院,江西省南昌市330006 [7]江西省卫生健康数字骨科重点实验室,江西省南昌市330006 [8]南方科技大学医院南方科技大学粤港澳智能与数字外科创新中心,广东省深圳市518000

出　　处：《中国组织工程研究》2023年第16期2493-2500,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81860384),课题名称:3D打印生物活性陶瓷大段人工骨的多尺度结构设计与降解特性调控,项目负责人:董谢平。

摘　　要：背景:目前的人工骨大多只能制备成小体积的填充用材料,大段负重骨及大块结构性植骨材料仍面临缺乏理想骨缺损修复材料和材料成型过程难以加工调控两大难题。目的:制备与人体大段负重骨形貌结构相似且兼具一定力学性能、优良生物相容性和部分可降解性的多孔羟基磷灰石/双相磷酸钙涂层复合支架,评估其性能。方法:采用水热合成法结合喷雾干燥技术分别制备羟基磷灰石粉体、羟基磷灰石晶须和β-磷酸三钙粉体,评估羟基磷灰石粉体、β-磷酸三钙粉体浸提液的细胞毒性。在羟基磷灰石粉体中添加不同含量(5%,10%,15%)的晶须,借助3D打印技术制备成多孔骨支架,设置不同的烧结工艺,通过正交实验筛选力学性能、孔隙率等最优的晶须含量与烧结工艺组合,检测最优组合支架的流体力学。采用浸渍提拉法将双相磷酸钙涂层涂覆在多孔骨支架上,体外评估双相磷酸钙涂层中β-磷酸三钙粉体的降解性能。结果与结论:①MTT实验显示,羟基磷灰石粉体、β-磷酸三钙粉体无明显的细胞毒性;②正交实验结果显示,影响多孔支架抗压强度最主要的因素为烧结温度,其次是晶须含量,最后是烧结时间;各因素组合中的最优组合为晶须含量10%、烧结温度1300℃、烧结时间3 h,此时支架抗压强度为8.13 MPa,孔隙率为64.07%;③通过流体力学模拟仿真可知,细胞营养液等垂直人工骨横截面方向流入流出的方式更有利于支架在人体中发挥作用;④在飞纳电镜的光学模式下,浸渍涂层前多孔支架的孔径为400μm,浸渍涂层后多孔支架的孔径为250μm,涂层厚度约为75μm;复合涂层中的β-磷酸三钙具有良好的体外降解性能;⑤结果显示,制备的羟基磷灰石/双相磷酸钙涂层复合支架具有较高的孔隙率与力学性能、优异的生物相容性和部分可降解性,是一种优良的生物活性陶瓷支架。BACKGROUND:At present,the majority of artificial bone can only be prepared into small-volume filling materials.Large sections of load-bearing bone and large-scale structural bone graft materials still face two major problems:lack of ideal bone defect repair materials and difficulty in material processing and regulating process.OBJECTIVE:To prepare the hydroxyapatite porous bone tissue scaffold with biphase calcium phosphate coating,which is similar to the morphology and structure of human large weight-bearing bone and has certain mechanical properties,excellent biocompatibility and partial degradability and assess its property.METHODS:Two different morphologies of hydroxyapatite(powder and whisker)andβ-tricalcium phosphate powder were prepared by hydrothermal synthesis method combined with spray drying technology to evaluate the cytotoxicity of hydroxyapatite powder andβ-tricalcium phosphate powder extract.Using 3D printing technology,the mixture of hydroxyapatite powder and whiskers(5%,10%,and 15%)was prepared into a porous bone embryo scaffold.Different sintering processes were set,and the optimal combination of whisker content and sintering process,such as mechanical properties and porosity,was screened through orthogonal experiments.The hydrodynamics of the optimal combination scaffold was tested.The biphase calcium phosphate coating was coated on the porous scaffold by dipping and pulling method to in vitro assess the degradation performance ofβ-tricalcium phosphate powder in biphase calcium phosphate coating.RESULTS AND CONCLUSION:(1)MTT assay showed that hydroxyapatite powder andβ-tricalcium phosphate powder did not have apparent cytotoxicity.(2)Orthogonal experiment results exhibited that sintering temperature,whisker content,and sintering time were the most important factors affecting the mechanical properties of the scaffold.The optimal parameters of the prepared scaffold were explored:sintering temperature of 1300℃,whisker content of 10%,sintering time of 3 hours.At this time,the compressive stre

关 键 词：力学性能 多孔支架 双相磷酸钙 羟基磷灰石晶须 3D打印 

分 类 号：R459.9[医药卫生—治疗学] R394.2[医药卫生—临床医学] R686.5