基于网络药理学探讨独活-羌活-细辛治疗膝骨关节炎的作用机制  

Mechanism of Angelicae Pubescentis Radix,Notopterygii Rhizoma et Radix,and Asari Radix et Rhizoma in Treatment of Knee Osteoarthritis Based on Network Pharmacology

作　　者：卢钰 保超宇 李具宝 尹本敬[1,2] 刘畅 邱荣能[2] 潘斯学[2] 何光雄 向俊宜 LU Yu;BAO Chaoyu;LI Jubao;YIN Benjing;LIU Chang;QIU Rongneng;PAN Sixue;HE Guangxiong;XIANG Junyi(Yunnan University of Chinese Medicine,Kunming 650500,China;Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China;The First People's Hospital of Yunnan Province,Kunming 650032,China)

机构地区：[1]云南中医药大学,云南昆明650500 [2]云南省中医医院,云南昆明650021 [3]云南省第一人民医院,云南昆明650032

出　　处：《云南中医学院学报》2022年第3期74-84,共11页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：云南省科技计划项目(202412)。

摘　　要：目的基于网络药理学的方法分析和预测独活-羌活-细辛治疗膝骨关节炎可能的作用机制。方法利用TCMSP数据库获取独活、羌活、细辛的活性成分及靶点,与CTD、NCBI等数据库获取的膝骨关节炎疾病基因靶点映射,构建药物-活性成分-疾病靶点网络。进行MCODE聚类分析,通过Omicshare云平台和DAVID数据库GO、KEGG通路富集分析。结果从3味药中共筛选出了36个活性成分及205个靶点,主要有山柰酚、β-谷甾醇、芝麻脂素、异紫花前胡苷等关键成分,AKT1、ESR1、JUN、CASP3等为关键靶点,核心基因有NOS3、ESR1、COX1、NR1I2,关键的生物进程和通路有类固醇激素反应性、活性氧代谢过程、AGE-RAGE通路和TNF通路。结论独活-羌活-细辛可能通过多成分、多靶点网络来治疗膝骨关节炎。Objective Based on the method of network pharmacology,the mechanism of Angelicae Pubescentis Radix-Notopterygii Rhizoma et Radix-Asari Radix et Rhizoma in the treatment of knee osteoarthritis was analyzed and predicted.Methods The TCMSP database was used to obtain the active ingredients and targets of Angelicae Pubescentis Radix-Notopterygii Rhizoma et Radix-Asari Radix et Rhizoma,and the gene targets of knee osteoarthritis obtained from CTD,NCBI and other databases were mapped to build a drug-active ingredient-disease target network.MCODE cluster analysis was carried out,and GO and KEGG pathway enrichment analysis was carried out through Omicshare cloud platform and DAVID database.Results A total of 36 active ingredients and 205 targets were screened from the three medicines.The key active compounds included kaempferol,β-sitosterol,sesamin,marmesinin and so on,the key targets included AKT1,ESR1,JUN,CASP3,etc.NOS3,ESR1,ACOX1 and NR1I2 are core genes.Steroid hormone responsiveness,reactive oxygen species metabolism,AGE-RAGE pathway and TNF pathway are the key biological processes and pathways.Conclusion Angelicae Pubescentis Radix,Notopterygii Rhizoma et Radix,Asari Radix et Rhizoma may treat knee osteoarthritis through multi-component and multi-target network.

关 键 词：网络药理学 膝骨关节炎 独活 羌活 细辛 

分 类 号：R285[医药卫生—中药学]