冈田酸诱导阿尔兹海默病动物模型的研究进展  

Research progress of okadaic acid induced Alzheimer′s disease animal model

作　　者：张鑫 王军燕 魏玉婷 刘鸿鑫 朱田田 严兴科 ZHANG Xin;WANG Junyan;WEI Yuting;LIU Hongxin;ZHU Tiantian;YAN Xingke(School of Acupuncture and Massage of Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu,730000)

机构地区：[1]甘肃中医药大学针灸推拿学院,甘肃兰州730000

出　　处：《实用临床医药杂志》2022年第19期129-134,共6页Journal of Clinical Medicine in Practice

基　　金：甘肃省高等学校青年博士基金项目(2021QB-072);甘肃省教育厅优秀研究生“创新之星”项目(2021CXZX-755);甘肃中医药大学引进人才科研启动基金项目(2019YJRC-05);甘肃中医药大学科学研究与创新基金项目(2022KCYB-1)。

摘　　要：冈田酸(OA)是一种强聚醚海洋毒素,能抑制蛋白磷酸酯酶-1(PP1)、蛋白磷酸酯酶-2A(PP2A)活性,促使tau蛋白过度磷酸化。根据该特性以及利用OA造模操作简单、成本低、造模周期短等特点,OA被广泛应用于国内外以tau蛋白磷酸化改变为特征的阿尔兹海默病(AD)模型制作中,但文献报道中OA的具体应用方法尚不统一。本文收集国内外相关研究,对实验动物选择、OA模型制备、注射部位、注射剂量、模型评价以及病理学机制进行综述,为该模型的合理应用提供参考。Okadaic acid(OA) is a strong polyether marine toxin, which can inhibit the activity of protein phosphatase-1(PP1) and protein phosphatase-2 A(PP2 A), and promote the hyperphosphorylated tau protein. Based on the characteristics of OA and its simple operation, low cost and short modeling cycle, OA has been widely used in the development of Alzheimer’s disease(AD) models characterized by changes of phosphorylated tau at home and abroad. However, the specific application methods of OA in literature reports are not uniform. This paper collected relevant researches at home and abroad, summarized the progress of OA in establishing AD model from the aspects of OA preparation, modeling animal species, intracerebral injection site, OA injection dose, model evaluation methods, and action mechanism, providing a reference for the rational application of the model.

关 键 词：阿尔茨海默病 动物模型 冈田酸 TAU蛋白 脑内注射 

分 类 号：R965[医药卫生—药理学] R818.02[医药卫生—药学]