EUCAST和CLSI微量肉汤稀释法白念珠菌体外药物敏感性试验结果比较  

Comparison of EUCAST and CLSI broth microdilution methods for the susceptibility testing against Candida albicans

作　　者：张亚茹 凌丽燕 陈敏 ZHANG Yaru;LING Liyan;CHEN Min(Department of Clinical Laboratory,the Second People's Hospital of Pinghu City,Pinghu 314201,Zhejiang,China;Department of Dermatology,the Second Affiliated Hospital of Naval Military Medical University,Shanghai 200003,China)

机构地区：[1]平湖市第二人民医院检验科,浙江平湖314201 [2]海军军医大学第二附属医院皮肤科,上海200003

出　　处：《检验医学》2022年第10期974-978,共5页Laboratory Medicine

基　　金：嘉兴市科学技术局公益性研究计划(2019AY32015);平湖市科技计划项目(平科持〔2020〕28号)。

摘　　要：目的比较欧洲抗菌药物敏感性试验委员会(EUCAST)和美国临床实验室标准化协会(CLSI)微量肉汤稀释法白念珠菌体外药物敏感性试验结果的差异。方法分别用EUCAST方法和CLSI方法检测42株白念珠菌对两性霉素B、阿尼芬净、卡泊芬净、氟康唑、伊曲康唑、伏立康唑、泊沙康唑和米卡芬净的敏感性,比较2种方法的基本一致性(EA)、分类一致性(CA)、极重大误差率(VME)和重大误差率(ME)。结果EUCAST方法和CLSI方法总体EA为95.2%~100.0%,CA为95.2%~100.0%,ME为0%~4.8%,VME为0%~2.4%;氟康唑的ME为4.8%,泊沙康唑的VME为2.4%。结论EUCAST方法和CLSI方法白念珠菌体外药物敏感性试验结果具有可比性,但部分药物之间需要额外的协调步骤。Objective To compare the results of susceptibility testing by European Committee on Antimicrobial Susceptibility Testing(EUCAST)and the Clinical and Laboratory Standards Institute(CLSI)broth microdilution methods against Candida albicans.Methods The susceptibilities of 42 isolates of Candida albicans were determined for amphotericin B,anidulafungin,caspofungin,fluconazole,itraconazole,voriconazole,posaconazole and micafungin according to EUCAST and CLSI methods.The essential agreement(EA),categorical agreement(CA),very major errors(VME)and major errors(ME)of the 2 methods were compared.Results The EA was 95.2%-100.0%.The CA,ME and VWE were 95.2%-100.0%,0%-4.8%and 0%-2.4%,respectively.The ME for fluconazole was 4.8%,and the VME for posaconazole was 2.4%.Conclusions The results of susceptibility testing by EUCAST and CLSI broth microdilution methods are comparable against Candida albicans.There are several areas where additional steps toward harmonization are warranted.

关 键 词：白念珠菌 微量肉汤稀释法 体外药物敏感性试验 欧洲抗菌药物敏感性试验委员会 美国临床实验室标准化协会 

分 类 号：R379.4[医药卫生—病原生物学]