蜈蚣败毒饮对寻常型银屑病患者(血热内蕴型)外周血树突状细胞分化影响的临床研究  

Clinical Study on Influence of Wugong Baidu Yin to DC Differentiation in Patients with Psoriasis Vulgaris of Blood-Heat Accumulation Syndrome

作　　者：郑毅[1] 王欣波[1] 刘庆南 杨素清[1] ZHENG Yi;WANG Xinbo;LIU Qingnan;YANG Suqing(The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China;Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中医药学报》2022年第11期60-64,共5页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金面上项目(81973846)。

摘　　要：目的:观察蜈蚣败毒饮对寻常型银屑病(血热内蕴证)患者外周血树突细胞及相关炎症因子的影响。方法:将72例寻常型银屑病(血热内蕴证)患者随机分为对照组(复方青黛胶囊组)36例、治疗组(蜈蚣败毒饮组)36例,另外选取10例健康受试者。采集患者和健康受试者的空腹外周血,分离单核细胞,进行RNA-seq高通量测序并鉴别银屑病患者与健康受试者的差异基因;治疗8周后,观察治疗组和对照组患者皮损面积及严重程度指数(PASI评分)变化;采用CCK-8法检测患者外周血T细胞的增殖活性,应用ELISA法和流式细胞术检测辅助性Th17细胞分化相关因子的变化。结果:(1)寻常型银屑病患者与健康受试者有31个差异基因,其中7个基因表达上调(KLK10、CD22、DYNAP、SIGLEC16、EBLN2、ERN2、SIGLEC11、KALRN),11个基因表达下调;(2)两组患者治疗前后比较,PASI评分均降低,且治疗组明显低于对照组(P<0.05);(3)治疗组与对照组相比,治疗后IL-6、IL-17含量均显著低于治疗前(P<0.05),且治疗组水平低于对照组,差异具有统计学意义(P<0.05);(4)两组对DC细胞的活力值治疗前后比较,差异有统计学意义(P<0.05),且治疗组优于对照组(P<0.05)。结论:蜈蚣败毒饮能有效缓解寻常型银屑病(血热内蕴证)的症状,降低PASI评分,并降低DCs的活性。Objective:To observe the effect of Wugong Baidu Yin on peripheral blood dendritic cells(DC)and related inflammatory factors in patients with psoriasis vulgaris of blood-heat accumulation syndrome.Methods:72 patients with psoriasis vulgaris of blood-heat accumulation syndrome were randomly divided into the control group and the treatment group,with 36 cases in each group.The control group was treated with Compound Qingdai Capsule,whereas the treatment group was treated with Wugong Baidu Yin.Peripheral blood was collected from fasting patients and from 10 healthy people,monocytes were isolated,and RNA-seq high-throughput sequencing was conducted to identify the genetic differences between psoriasis patients and healthy people.After 8 weeks of treatment,the changes of skin lesion area and severity index(PASI score)were observed in the two groups.The proliferative activity of peripheral blood T cells was detected by CCK-8 method,and the changes of helper Th17 cell differentiation related factors were detected by ELISA and flow cytometry.Results:There were 31 gene differences between patients with psoriasis vulgaris and healthy people,of which 7 genes were up-regulated(KLK10,CD22,DYNAP,SIGLEC16,EBLN2,ERN2,SIGLEC11,KALRN),and 11 genes were down-regulated.The PASI score was decreased after the treatment compared with that before the treatment in the two groups,and the PASI score of the treatment group was significantly lower than that of the control group after the treatment(P<0.05).The levels of IL-6 and IL-17 were lower after the treatment than those before the treatment in the two groups(P<0.05),and the levels of IL-6 and IL-17 in the treatment group were lower than those in the control group after the treatment(P<0.05).There were statistical differences in DC absorbance rate and OD value measured by CCK-8 between before and after the treatment in the two groups(P<0.05),of which the treatment group was better than the control group after the treatment(P<0.05).Conclusion:Wugong Baidu Yin can effectively alleviate the

关 键 词：蜈蚣败毒饮 寻常型银屑病 血热内蕴 树突状细胞 

分 类 号：R275.9[医药卫生—中西医结合] R758.63[医药卫生—中医皮科]