抗根肿病结球甘蓝Ogura CMS种间恢复材料的创制  

Creation of Cabbage Interspecific Material with Clubroot Resistance and Ogura CMS Restorer

作　　者：杨鼎 吕凤仙 李崇娟 徐学忠[2,3] 胡靖锋[2,3] 杨红丽[2,3] 兰梅[2,3] 张丽琴[2,3] 董相书 和江明[2,3] YANG Ding;LYU Fengxian;LI Chongjuan;XU Xuezhong;HU Jingfeng;YANG Hongli;LAN Mei;ZHANG Liqin;DONG Xiangshu;HE Jiangming(Yunnan University,Kunming 650504,Yunnan,China;Horticultural Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,Yunnan,China;Yunnan Branch of National Vegetable Improvement Center,Kunming 650205,Yunnan,China)

机构地区：[1]云南大学,云南昆明650504 [2]云南省农业科学院园艺作物研究所,云南昆明650205 [3]国家蔬菜改良中心云南分中心,云南昆明650205

出　　处：《中国蔬菜》2022年第11期44-52,共9页China Vegetables

基　　金：国家大宗蔬菜产业技术体系昆明综合试验站项目(CARS-23-G33);云南省“万人计划”产业领军人才项目(YNWRCYJS-2018-040);2022年云南省通海县蔬菜科技特派团项目(202204BI090023);绿色食品品牌打造科技支撑行动(果蔬)专项经费资助项目。

摘　　要：为获得抗根肿病且携带恢复基因的结球甘蓝Ogura CMS种间材料。以抗根肿病结球甘蓝品种先甘366为母本,以甘蓝型油菜Ogura CMS恢复材料N21015为父本,通过远缘杂交结合胚挽救获得种间杂种。抗根肿病表型鉴定和分子标记鉴定结果表明,杂种F_(1)对根肿病表现为免疫,具有母本的根肿病抗性基因和父本的Ogura CMS恢复基因;人工诱导染色体加倍后成功获得异源六倍体种间杂种,花粉可育率为78.46%。In order to obtain cabbage Ogura CMS interspecific material resistant to clubroot disease and carrying restorer gene,this experiment took cabbage‘Xiangan 366’resistant to clubroot as female parent and Ogura CMS restorer material Brassica napus L.‘N21015’as male parent;carried out distant hybridization combined with embryo rescue.Then the interspecific hybrids were obtained.The results of clubroot resistance phenotypic identification and molecular marker identification showed that hybrid F_(1) was immune to clubroot disease,and had the maternal clubroot resistance gene and paternal Ogura CMS restorer gene.After artificially induced chromosome doubling,heterohexaploid interspecific hybrids were successfully obtained,and the pollen fertility rate was 78.46%.

关 键 词：结球甘蓝 甘蓝型油菜 根肿病 Ogura CMS 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]