不同热处理方式脱除草莓植株体内草莓轻型黄边病毒的研究  

Research on Eliminating of Strawberry Mild Yellow Edge Virus in Fragaria ananassa by Different Heat Treatment Methods

作　　者：刘羽丰 闫哲 王志 封林林 于翠 任俊达 尚巧霞[1] LIU Yufeng;YAN Zhe;WANG Zhi;FENG Linlin;YU Cui;REN Junda;SHANG Qiaoxia(College of Bioscience and Resource Environment,Beijing College of Agriculture,Key Laboratory of Urban Agriculture(North China),Ministry of Agriculture and Rural Affairs,Beijing 102206,China;Cathay Green Seeds(Beijing)Co.,Ltd,Beijing 102200,China)

机构地区：[1]北京农学院生物与资源环境学院,农业农村部华北都市农业重点实验室,北京102206 [2]北京开心格林农业科技有限公司,北京102200

出　　处：《中国蔬菜》2022年第11期92-97,共6页China Vegetables

基　　金：北京市教委科技重点项目(KZ202210020028);北京农学院青年教师科研创新能力提升计划项目(QJKC2022002)。

摘　　要：2014—2022年对红颜草莓的组培苗进行了RT-PCR检测,发现其主要受草莓轻型黄边病毒(SMYEV)、草莓镶脉病毒(SVBV)、草莓斑驳病毒(SMoV)、草莓皱缩病毒(SCV)和黄瓜花叶病毒(CMV)危害,阳性样品的总检出率为20.2%。2022年组培苗中仅检测出SMYEV和SVBV,以携带上述病毒的红颜匍匐茎为试材进行组织培养,SMYEV的脱除率为25.0%,SVBV的脱除率为100.0%。为进一步确定脱除SMYEV的有效方法,对带毒茎尖组培苗进行热处理,并通过RT-PCR和RT-qPCR检测病毒脱除率。结果表明,3种热处理方式均能100.0%脱除红颜茎尖组培苗中的SMYEV。综合组培苗存活率和病毒脱除率,确定起始温度32℃,按1℃·d^(-1)升至38℃后培养30 d是最适宜的SMYEV脱除方式。The commercially avalable tissue cultured seedlings of strawberry variety‘Benihoppe’were detected by RT-PCR method from 2014 to 2022.It was found that they were mainly infected by strawberry mild yellow edge virus(SMYEV),strawberry vein banding virus(SVBV),strawberry mottle virus(SMoV),strawberry crinkle virus(SCV)and cucumber mosaic virus(CMV).The total detection rate of positive samples was 20.2%.Only SMYEV and SVBV were detected in tissue cultured seedlings in 2022.Taking the stolons of‘Benihoppe’carrying SMYEV or SVBV virus as test material,this experiment conducted tissue culture.The elimination rate of SMYEV was 25.0%and that of SVBV was 100.0%.In order to further determine the effective method for SMYEV elimination in strawberries,the veneniferous shoot-tip tissue culture seedlings were detoxified by thermotherapy treatment.The virus removal rate was detected by RT-PCR and RTqPCR.The results showed that all 3 thermotherapy treatments could completely(100.0%)eliminate SMYEV from shoot-tip of tissue culture seedlings.Comprehensively considering the survival rate and virus removal rate of tissue culture seedlings,thermotherapy for 30 days after the temperature from 32℃increase 1℃one day to 38℃is the most suitable way for SMYEV elimination.

关 键 词：草莓 热处理 脱毒 草莓轻型黄边病毒 病毒检测 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]