水蕨愈伤胚胎发生的代谢组学研究  

Metabolomes of Callus Embryogenesis in Ceratopteris richardii

作　　者：李贵生[1] LI Guisheng(College of Biological Resources and Environmental Sciences,Jishou University,Jishou,Hunan 416000,China)

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《西北植物学报》2022年第9期1523-1529,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金地区基金(32160150);湖南省教育厅项目(14K077)。

摘　　要：体细胞胚胎发生是植物繁殖的一种方式,而离体的愈伤胚胎发生既可以进行植株再生、又可以用于遗传转化。该研究采用非靶向代谢组学,对蕨类植物水蕨分别处于增殖期和分化期的愈伤进行代谢物差异分析,以探讨愈伤胚胎发生的代谢机制,为愈伤胚胎发生的代谢组学提供资料。结果发现:(1)水蕨增殖期与分化期的愈伤具有相同的代谢物,且其中一些为疾病药物如放线菌酮和三七素。(2)各代谢物的含量高低不一,但基本上在增殖期和分化期之间无差异。(3)增殖期积累的代谢物预测与ABC通道蛋白途径有关,而分化期积累的代谢物可能与黄酮合成途径有关。研究推测,水蕨愈伤增殖期到分化期的转变是代谢物的量变过程,而非质变;植物愈伤胚胎发生的代谢组可能具有物种特异性。Somatic embryogenesis is one mode of reproduction in plants,whereas ex vivo callus embryo-genesis can perform plant regeneration,but also can be employed for genetic transformation.This study employed the widely targeted metabolomics and explored respectively proliferating and differentiating calli in the fern Ceratopteris richardii,aiming to investigate metabolic mechanisms for callus embryogenesis and thus providing materials for metabonomics of callus embryogenesis.The results found:(1)both the proliferating and the differentiating callus possessed the same metabolites,and there were some disease drugs among them such as actidione and 3-[(Carboxycarbonyl)amino]-L-alanine.(2)Each metabolites had different contents,but basically they were not altered between the proliferative and the differentiating stages.(3)Metabolites accumulated in the proliferating stage were predicted related to the ABC transport-er protein pathway,while those in the differentiating stage probably concerning the flavonoid biosynthesis pathway.The research speculated transition from proliferating to differentiating phase of calli in C.rich-ardii was a quantitative rather than a qualitative process in terms of metabolite alterations,and metabo-lomes of plant callus embryogenesis might be characterized by species specificity.

关 键 词：愈伤胚胎发生 代谢组 黄酮 PLS-DA 药物 

分 类 号：Q789[生物学—分子生物学] Q945.6Q949.36