基于SSR分子标记的中国黄连木遗传多样性分析  

Genetic Diversity Analysis of Pistacia chinensis Bunge Based on SSR Markers

作　　者：任重 白倩[1,2] 苏淑钗 REN Zhong;BAI Qian;SU Shuchai(Ministry of Education Key Laboratory of Silviculture and Conservation,College of Forestry,Beijing Forestry University,Beijing 100083,China;National Energy R&D Center for Non-food Biomass,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林学院,省部共建森林培育与保护教育部重点实验室,北京100083 [2]北京林业大学,国家能源非粮生物质原料研发中心,北京100083

出　　处：《西北植物学报》2022年第9期1530-1539,共10页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家科技基础资源调查专项(2019FY100803)。

摘　　要：该研究利用筛选出的7对SSR引物,对中国20个省、市、自治区的210份种质资源进行分子标记试验,分析中国黄连木种质资源遗传多样性、亲缘关系、遗传分化特点并构建DNA分子身份证,为黄连木的资源保护、种质利用提供理论依据,结果表明:(1)7对引物在210份种质中共扩增出158个等位基因位点,平均每对引物的等位基因数为22.571个。(2)基因多样性(GD)变化幅度为0.654~0.913,平均为0.804;期望杂合度(He)变化范围0.257~0.771,平均为0.532;多态信息含量(PIC)变化范围0.639~0.907,平均为0.784。(3)从不同地区黄连木群体的遗传多样性来看,观测杂合度(Ho)介于0.373~0.600之间,平均值为0.520;期望杂合度(He)介于0.632~0.811之间,平均值为0.737;从各群体间遗传分化指数(Fst)来看,黄连木各地区群体间的遗传分化值在0.015~0.099之间,各群体间的遗传分化处于中等以下水平。(4)分子方差分析(AMOVA)结果显示,黄连木的遗传分化变异以群体内为主,占总变异量的94%,群体间的变异占6%。(5)UPGMA聚类、群体遗传结构分析和PCoA分析结果相一致,全部种质被划分为两大类,西南地区群体单独为一类,其他地区单独为一类。(6)利用7对SSR引物构建了210份黄连木种质的DNA分子身份证。In this test,seven pairs of SSR primers were selected to carry out molecular marker tests on 210 germplasm resources from 20 provinces,cities and autonomous regions in China,for studying the genetic diversity,kinship and genetic differentiation characteristics of the germplasm resources of Pistacia chinensis Bunge and constructing the DNA molecular identity card.It provides theoretical basis for the conservation and germplasm utilization of P.chinensis.The results showed that:(1)seven pairs of primers amplified 158 alleles from 210 germplasm,with an average number of 22.571 alleles per pair of primers.(2)The variation range of gene diversity(GD)was 0.654-0.913 with an average of 0.804.The expected heterozygosity(He)ranged from 0.257-0.771,with an average of 0.532.Polymorphic information content(PIC)ranged from 0.639-0.907,with an average of 0.784.(3)In terms of genetic diversity,the observed heterozygosity(Ho)ranged from 0.373-0.600,with an average of 0.520.The expected heterozygosity(He)was between 0.632-0.811,with an average of 0.737.According to the genetic differentia-tion index(Fst),the genetic differentiation value between populations in different regions of P.chinensis was between 0.015-0.099,and the genetic differentiation among populations was below the medium lev-el.(4)Molecular variance analysis(AMOVA)showed that the genetic differentiation variation of P.chinensis was mainly within the population,accounting for 94%of the total variation,and the inter-popu-lation variation accounted for 6%.(5)UPGMA clustering,PCoA analysis and population genetic struc-ture analysis results were consistent,all germplasm were divided into two groups,the southwest popula-tion was a separate group,the other regions were a separate group.(6)Seven pairs of SSR primers were used to construct 210 DNA identification cards of P.chinensis germplasm.

关 键 词：黄连木 SSR 遗传多样性 遗传分化 分子身份证 

分 类 号：Q789[生物学—分子生物学] Q949.93Q949.754.4