点按脾经合穴对急性钝挫伤大鼠骨骼肌炎症因子及钙离子稳态的影响  

Effects of point-pressing of the spleen meridian on inflammatory factors and calcium homeostasis in the skeletal muscle of acute blunt contusion rats

作　　者：刘海潮 王浩 王诗忠 林建平 金京 陈少清[1] Liu Haichao;Wang Hao;Wang Shizhong;Lin Jianping;Jin Jing;Chen Shaoqing(Rehabilitation School of Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,Fujian Province,China;School of Health,Fujian Medical University,Fuzhou 350122,Fujian Province,China)

机构地区：[1]福建中医药大学康复医学院,福建省福州市350122 [2]福建医科大学健康学院,福建省福州市350122

出　　处：《中国组织工程研究》2023年第20期3123-3128,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年项目(81904317)“基于内质网应激-自噬反应研究“脾主肌肉”理论下推拿脾经治疗骨骼肌损伤的作用机制”,项目负责人:林建平;国家自然科学基金面上项目(81973924)“基于外泌体miR-146a介导Akt/NF-κB通路探讨针刺委中穴治疗腰痛的作用机制”,项目负责人:陈少清。

摘　　要：背景:既往研究表明,推拿可促进骨骼肌损伤的修复,但其具体机制尚不明确。目的:探讨点按脾经合穴对急性钝挫伤大鼠骨骼肌修复过程中炎症和钙离子稳态的影响。方法:将32只雄性SD大鼠随机分为空白组、模型组、点按非穴组、点按穴位组,除空白组外,其余3组均建立急性腓肠肌钝挫伤模型。点按非穴组取阴陵泉同一水平线与胆经和膀胱经中线的相交点穴;点按穴位组取“阴陵泉”穴,均1次/d,连续治疗7 d。干预结束后采用Catwalk小动物步态分析系统观察各组大鼠运动情况,苏木精-伊红染色法观察骨骼肌形态变化,ELISA法检测炎症因子水平和内质网钙ATP酶的活性,钙含量显色法检测骨骼肌组织钙浓度的变化,TUNEL法检测骨骼肌组织内细胞凋亡水平。结果与结论:①与模型组比较,点按穴位组的平均支撑时相显著增大(P<0.01);与点按非穴组比较,点按穴位组平均支撑时相显著增大(P<0.05);②点按穴位组炎性细胞和红细胞聚集情况减少,且骨骼肌修复情况优于点按非穴组;③与模型组相比,点按穴位组肿瘤坏死因子α、白细胞介素6的质量浓度显著降低(P<0.01),SERCA活性显著提高(P<0.01);与点按非穴组比较,点按穴位组肿瘤坏死因子α质量浓度显著降低(P<0.01),白细胞介素6的变化无显著差异(P>0.05),SERCA的活性显著提高(P<0.01);④与模型组比较,点按穴位组Ca^(2+)浓度显著降低(P<0.01);与点按非穴组相比,点按穴位组Ca^(2+)的浓度显著降低(P<0.05);⑤与模型组比较,点按非穴组与点按穴位组细胞凋亡率显著降低(P<0.01);与点按非穴组比较,点按穴位组细胞凋亡率显著降低(P<0.05);⑥提示点按脾经合穴可有效改善骨骼肌损伤大鼠运动功能,促进骨骼肌修复,其机制可能与降低炎症因子、促进钙泵功能、维持钙内环境稳态、从而减少细胞凋亡相关。BACKGROUND:Previous studies have shown that tuina can promote the repair of skeletal muscle injuries,but the specific mechanism is unclear.OBJECTIVE:To investigate the effect of point-pressing of the spleen meridian on inflammation and calcium homeostasis in skeletal muscle repair in rats with acute blunt contusion injuries.METHODS:Thirty-two male Sprague-Dawley rats were randomly divided into blank group,model group,non-acupoint percussion group,and acupoint percussion group.Except for the blank group,animal models of acute gastrocnemius blunt contusion were established in the other three groups.In the nonacupoint pressing group,the intersection point of the same horizontal line of Yinlingquan acupoint(SP 9)and the midline of the gallbladder and bladder meridians was taken for percussion.In the acupoint pressing group,percussion was performed at the Yinlingquan acupoint.Point-pressing in the two groups was done once a day,for 7 consecutive days.Catwalk small animal gait analysis system was used to observe locomotor activity of rats.Hematoxylin-eosin staining was performed to observe the skeletal muscle morphology changes.ELISA method was used to detect the levels of inflammatory factors and the activity of sarcoplasmic/endoplasmic reticulum Ca^(2+)ATPase(SERCA).Calcium concentration in the skeletal muscle was measured using the calcium assay.The level of apoptosis in the skeletal muscle was detected by TUNEL method.RESULTS AND CONCLUSION:Compared with the model and non-acupoint pressing groups,the average support phase was significantly increased in the acupoint pressing group(P<0.01,P<0.05).Aggregation of inflammatory cells and red blood cells was reduced in the acupoint percussion group,and skeletal muscle repair was also better in the acupoint percussion group than the non-acupoint pressing group.Compared with the model group,the levels of tumor necrosis factorαand interleukin-6 were significantly decreased in the acupoint pressing group(P<0.01),and the SERCA activity was significantly increased(P<0.01).Comp

关 键 词：骨骼肌 钝挫伤 钙稳态 钙泵 炎症因子 细胞凋亡 点按 脾主肌肉 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R274.3