通腑逐瘀法指导下抵当汤加减可抑制大鼠急性脊髓损伤后胶质瘢痕的形成  

Jiajian Didang Tang inhibits glial scar formation in rats with acute spinal cord injury under the guidance of Tongfu Zhuyu therapy

作　　者：文峰[1,2,3,4] 周磊 李扬[2,3,4] 郑力铭 张志文 范筱 吴子健 Wen Feng;Zhou Lei;Li Yang;Zheng Liming;Zhang Zhiwen;Fan Xiao;Wu Zijian(Hubei University of Chinese Medicine,Wuhan 430065,Hubei Province,China;Affiliated Hospital of Hubei University of Chinese Medicine,Wuhan 430061,Hubei Province,China;Hubei Provincial Hospital of TCM,Wuhan 430061,Hubei Province,China;Hubei Academy of TCM,Wuhan 430061,Hubei Province,China;Qingdao Municipal Hospital,Qingdao 266011,Shandong Province,China)

机构地区：[1]湖北中医药大学,湖北省武汉市430065 [2]湖北中医药大学附属医院,湖北省武汉市430061 [3]湖北省中医院,湖北省武汉市430061 [4]湖北省中医药研究院,湖北省武汉市430061 [5]青岛市市立医院,山东省青岛市266011

出　　处：《中国组织工程研究》2023年第20期3180-3187,共8页Chinese Journal of Tissue Engineering Research

基　　金：湖北省卫健委中医药科研项目(ZY2019M044),项目参与人:吴子健。

摘　　要：背景:急性脊髓损伤具有较高的致残率和致死率,在急性期进行积极有效的治疗对预防继发性损害具有重要意义。目的:观察通腑逐瘀法指导下抵当汤加减治疗大鼠急性脊髓损伤后水肿和肢体功能恢复的疗效,探讨通腑逐瘀法治疗急性脊髓损伤的作用机制。方法:将60只SD大鼠随机分为假手术组、模型组、抵当汤组、TGN-020组以及抵当汤+TGN-020组,每组12只。假手术组行椎板切除术,不予脊髓打击,其余各组均采用纽约大学打击器制造急性脊髓损伤模型。造模成功后,假手术组和模型组不采取任何治疗措施,抵当汤组术后给予5.04 g/kg抵当汤灌胃;TGN-020组术后给予5 mg/kg TGN-020(水通道蛋白4特异性抑制剂)腹腔注射;抵当汤联合TGN-020组术后采用5 mg/kg TGN-020腹腔注射联合5.04 g/kg抵当汤灌胃处理,1次/d,持续7 d。分别于术后1,3,5,7天评估BBB评分和Reuter评分,术后第7天取材进行脊髓组织大体观察和含水率检测,苏木精-伊红染色观察组织受损程度,免疫荧光检测各组脊髓组织中水通道蛋白4和胶质原纤维酸性蛋白共表达细胞个数,Western Blot法检测各组脊髓组织中水通道蛋白4、胶质原纤维酸性蛋白、硫酸软骨素蛋白聚糖、增殖细胞核抗原蛋白的表达量。结果与结论:(1)行为学评分结果显示,与模型组相比,各用药组BBB评分均显著升高(P<0.05),Reuter评分均显著降低(P<0.05),且各用药组之间无显著差异(P>0.05);(2)脊髓组织含水率结果显示,与模型组相比,各用药组脊髓组织含水率均显著降低(P<0.05),且各用药组之间无显著差异(P>0.05);(3)苏木精-伊红染色结果显示,模型组脊髓组织结构离乱,大量瘢痕组织和脊髓空洞形成;与模型组相比,各用药组可见组织结构较完整,可见少量瘢痕组织增生和脊髓空洞,组织形态得到有效改善;(4)免疫荧光结果显示,与模型组相比,各用药组水通道蛋白4和胶质原纤维酸性蛋BACKGROUND:Acute spinal cord injury is a disease with high disability and mortality.An active and effective treatment in the acute phase is of great significance to prevent secondary injury.OBJECTIVE:To investigate the mechanism underlying Tongfu Zhuyu therapy in the treatment of acute spinal cord injury through observing the therapeutic effect of Jiajian Didang Tang on edema and recovery of limb function after acute spinal cord injury in rats.METHODS:A total of 60 Sprague-Dawley rats were randomly divided into sham group,model group,Didang Tang group,2-(nicotinamide)-1,3,4-thiadiazole(TGN-020)group,and Didang Tang combined with TGN-020 group(combined group),with 12 rats in each group.Among them,the sham group only underwent laminectomy without spinal cord shock and the other groups used NYU percussion device to create an acute spinal cord injury model.After modeling,the sham and model groups did not take any treatment measures.The Didang Tang group was given 5.04 g/kg Didang Tang by intragastric administration,the TGN-020 group was given 5 mg/kg TGN-020 by intraperitoneal injection,and the combined group was given TGN-020 by intraperitoneal injection combined with Didang Tang by intragastric administration,once a day for 7 days.Basso,Beattie and Bresnahan score and Reuter score were evaluated on 1,3,5 and 7 days after operation.Spinal cord tissue was taken for general observation and water content measurement at 7 days after operation.The degree of tissue damage was detected by hematoxylin-eosin staining.The number of aquaporin 4/glial fibrillary acidic protein-positive cells in the spinal cord tissue was detected by immunofluorescence assay.The protein expression levels of Aquaporin 4,glial fibrillary acidic protein,chondroitin sulfate proteoglycan,and proliferating cell nuclear antigen protein in spinal cord tissue were detected by western blot.RESULTS AND CONCLUSION:The behavioral scores showed that compared with the model group,the Basso,Beattie and Bresnahan scores were significantly increased in the three

关 键 词：通腑逐瘀法 抵当汤 急性脊髓损伤 水肿 瘢痕 组织增生 星形胶质细胞 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R274