环境因子和储藏时期对长枝木霉PT6蛋白培养滤液杀线活性评价  

Nematicidal activity of Trichoderma longibrachiatum PT6 protein culture filtrate under different environmental factors and storage periods

作　　者：刘永红 毛维兴 陈瑞莲 马耀杰 张树武 徐秉良 LIU Yonghong;MAO Weixing;CHEN Ruilian;MA Yaojie;ZHANG Shuwu;XU Bingliang(College of Plant Protection,Gansu Agricultural University/Engineering Laboratory for Biological Control of Crop Diseases and Pests,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学植物保护学院/甘肃省农作物病虫害生物防治工程实验室,甘肃兰州730070

出　　处：《草业科学》2022年第10期2095-2101,共7页Pratacultural Science

基　　金：甘肃农业大学“伏羲杰出人才培育计划”项目(Gaufx-03J03);甘肃农业大学人才专项经费(2017RCZX-07);国家自然基金(31860526);甘肃省杰出青年基金(18JR3RA161)。

摘　　要：为了明确长枝木霉(Trichoderma longibrachiatum)PT6蛋白培养滤液对禾谷孢囊线虫(Heterodera avenae)2龄幼虫的毒杀活性及其稳定性。通过室内杀线活性测定,分析和评价了不同环境因子和储藏时期对长枝木霉PT6蛋白培养滤液杀线活性的影响。结果表明,不同pH、金属离子、氧化剂、温度、还原剂和储藏时期对PT6蛋白培养滤液杀线活性和稳定性都有不同的影响。pH 4~10时,PT6蛋白培养滤液对禾谷孢囊线虫2龄幼虫具有较高毒杀作用,处理72 h后其死亡率均大于80%,且与原始蛋白培养滤液(对照)相比差异性不显著(P>0.05);在温度为30~60℃条件下与对照相比,杀线活性无显著差异(P>0.05),当温度超过70℃时,杀线活性开始降低;Cu^(2+)对PT6蛋白培养滤液杀线活性具有显著抑制效果(P<0.05),而K^(+)和Zn^(2+)对其杀线活性具有显著增强作用(P<0.05),Fe^(3+)、Mg^(2+)、Mn^(2+)和Na^(+)对PT6蛋白培养滤液杀线活性无显著影响(P>0.05);当氧化剂浓度低于0.08 mol·L^(−1)、还原剂低于0.06 mol·L^(−1)时,对杀线活性无显著影响(P>0.05);与对照相比,当PT6蛋白培养滤液在室温下保存0~60 d,其杀线活性无显著变化(P>0.05)。综上所述,PT6蛋白培养滤液对禾谷孢囊线虫具有较强的毒杀作用,且稳定性较好。To determine the toxic activity and stability of Trichoderma longibrachiatum PT6 protein culture filtrate against second stage juveniles(J2s)of Heterodera avenae,the in vitro nematicidal activity of PT6 protein culture filtrate was analyzed and evaluated under different environmental factors and storage periods.Different pH,temperature,metal ions,redox concentrations,and storage periods presented different effects on the nematicidal activity and stability of the PT6 protein culture filtrate.The PT6 protein culture filtrate had a high lethal effect on J2s of the cereal cyst nematode,with more than 80%mortality with treatment at pH 4~10 for 72 h,and no significant difference compared with the control.At the temperature of 30~60℃,there was no significant difference in the nematicidal activity of PT6 protein culture filtrate in comparison to the control.The nematicidal activity decreased significantly at temperatures higher than 70℃.Zn^(2+) and K^(+)clearly enhanced the nematicidal activity of PT6 protein culture filtrate,whereas Cu^(2+) inhibited the nematicidal activity.However,Mg^(2+),Fe^(2+),Mn^(2+),and Na^(+)had no significant effect on the nematicidal activity of PT6 protein culture filtrate.At an oxidant concentration lower than 0.08 mol·L^(−1) and reducing agent concentration lower than 0.06 mol·L^(−1),no significant effect was observed on the nematicidal activity of J2s of H.avenae.Storage of the culture filtrate at room temperature for 0~60 days did not result in significant changes in nematicidal activity.Therefore,the PT6 protein culture filtrate had a strong lethal effect on the cereal cyst nematode,and its nematicidal activity was stable.

关 键 词：杀线活性 稳定性 温度 酸碱度 金属离子 氧化剂 还原剂 

分 类 号：TQ450.1[化学工程—农药化工]