选择适用于研究滋养层细胞合体化过程的内参基因的实验研究  

Selection of internal references gene for studying trophoblast syncytialization

作　　者：郑婉珊 殷茵[3] 涂兆伟 曹彬 Zheng Wanshan;Yin Yin;Tu Zhaowei;Cao Bin(Department Obstetrics and Gynecology,The Third Affiliated Hospital of Guangzhou Medical University,Guangdong Province Key Laboratory of Major Obstetric Diseases,Guangzhou Medical Center for Critical Pregnant Women,Guangzhou 510150,China;Fujian Provincial Key Laboratory of Reproductive Health Research,School of Medicine Xiamen University,Xiamen 361102,China;Department of Obstetrics and Gynecology,The First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]广州医科大学附属第三医院妇产科广东省产科重大疾病重点实验室广州重症孕产妇救治中心,510150 [2]厦门大学医学院生殖调控与生殖健康研究福建省高等学校重点实验室,361102 [3]南京医科大学附属第一医院妇产科,210029

出　　处：《中华产科急救电子杂志》2022年第1期46-52,共7页Chinese Journal of Obstetric Emergency（Electronic Edition）

基　　金：国家重点研发计划项目(2018YFC1004400);国家自然科学基金项目(81971414);福建省自然科学基金项目(2020J06003)。

摘　　要：目的筛选并确定适用于滋养层细胞合体化研究的内参基因。方法利用人绒毛膜癌细胞BeWo和人滋养细胞干细胞比较了11个候选内参基因的稳定性,并使用GeNorm、NormFinder和BestKeeper三种软件程序来确定最合适用于研究滋养层细胞合体化的内参基因。结果TBP和RPS13为稳定表达的内参基因,而常用的GAPDH(glyceraldehyde-3-phosphate dehydrogenase)和β-actin(actin,beta)在滋养层细胞合体化过程中表达水平稳定性不佳。结论在利用实时荧光定量聚合酶链反应进行滋养层细胞的合胞化研究时,TBP和RPS13是首选的内参基因。Objective To screen and identify reference genes suitable for studying on trophoblast cells.Methods The stability of 11 candidate reference genes were compared using BeWo and human trophoblast stem cells(hTSCs).Three software programs,GeNorm,NormFinder,and BestKeeper,were used to determine the most suitable internal reference genes for the study of trophoblast cell syncytialization.Results TBP and GRPS13 were stable reference genes,while GAPDH andβ-actin expression levels were not stable during trophoblast cell syncytialization.Conclusions TBP and RPS13 were recommended as the preferred internal reference genes for trophoblast syncytialization studies using RT-PCR.

关 键 词：滋养层 细胞系 细胞融合 聚合酶链反应 

分 类 号：Q253[生物学—细胞生物学]