仿生树叶模型的制作及在琼脂糖微流控芯片中的应用  

Fabrication of Bionic Leaf Model and Its Application in Agarose Microfluidic Chip

作　　者：王方圆 张奋娴 李毅 高建华[1] 牛颜冰[1] 申少斐 WANG Fangyuan;ZHANG Fenxian;LI Yi;GAO Jianhua;NIU Yanbing;SHEN Shaofei(College of Life Sciences,Shanxi Agricultural University,Shanxi Key Lab for Modernization of Traditional Chinese Veterinary Medicine,Taigu 030801,China)

机构地区：[1]山西农业大学生命科学学院,中兽医药现代化山西省重点实验室,太谷030801

出　　处：《高等学校化学学报》2022年第11期53-58,共6页Chemical Journal of Chinese Universities

基　　金：国家青年自然科学基金(批准号:31700749);山西省基础研究计划项目(批准号:20210302123368)资助。

摘　　要：树叶的脉序可以更好模拟人体血管微环境,但以自然环境中树叶为模板进行实验存在一定的季节局限性;且不能保证每次实验所用树叶脉序形状相同,不易控制变量,且会对环境造成一定破坏.本文建立了一种简易的仿生树叶模型制作方法,并通过仿生模型构建了经济、易操作的琼脂糖微流控芯片.分别测定了芯片的一、二、三级脉序数目及尺寸,最大脉序尺寸值可达1038.02μm,最小脉序尺寸为36.32μm,宽度不一的通道构建为细菌趋化性实验提供了稳定可靠的梯度空间,对探索药物筛选、微生物利害等研究具有重要意义.The vein sequence of leaf is similar with human vascular network in terms of structure and function,highlighting a manner to create human vessel-like structure in vitro.However,several technical(irreproducible vein pattern of different leaves)and logistic(limited availability across seasons)issues prevent the use of fresh leaves as template.Herein,a practical and effective method for biomimetically replicating the leaf structure was developed and this bionic leaf model was applied to constructing a low-cost and simple-to-operate agarose microfluidic chip.Moreover,the number and size of the pulse sequences(channels)of the primary veins,secondary veins and tertiary veins in the agarose chip were measured,where maximum and minimum widths of pulse sequence are 1038.02μm and 36.32μm,respectively.Lastly,the fabricated channels of different widths provided stable and reliable gradients for studying bacterial chemotaxis.Taken together,our methodology is of great significance for drug screening and microbial research.

关 键 词：仿生 微流控芯片 趋化性 

分 类 号：O657[理学—分析化学]