大菱鲆和红鳍东方鲀脂肪酸转运蛋白的组织分布及营养调控  

Tissue Distribution and Nutritional Regulation of Fatty Acid Transport Protein in Scophthalmus maximus and Takifugu rubripes

作　　者：赵丽丽 王迪欣 廖章斌 毕清竹 马强 卫育良 梁萌青[2] 乔秀亭 程镇燕 徐后国[2] ZHAO Lili;WANG Dixin;LIAO Zhangbin;BI Qingzhu;MA Qiang;WEI Yuliang;LIANG Mengqing;QIAO Xiuting;CHENG Zhenyan;XU Houguo(College of Fisheries,Tianjin Agricultural University,Tianjin 300392,China;Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China)

机构地区：[1]天津农学院水产学院,天津300392 [2]中国水产科学研究院黄海水产研究所,青岛266071

出　　处：《动物营养学报》2022年第10期6620-6633,共14页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：中央公益性科研机构基础研究基金项目(20603022022004,2020TD48);财政部和农业农村部农业产业技术体系(CARS-47)。

摘　　要：本研究对2种海洋硬骨鱼(大菱鲆和红鳍东方鲀)脂肪酸转运蛋白(FATP)亚型FATP1、FATP4和FATP6进行了序列分析,并对其在不同组织或不同营养状况下的基因表达特征进行了研究。在FATP表达的组织分布研究中,分别取大菱鲆和红鳍东方鲀的不同组织。在饲养试验中,分别饲喂大菱鲆和红鳍东方鲀幼鱼不同脂肪水平(8%、12%和16%)的试验饲料,进行9周的饲养试验,然后进行饥饿试验(30 d)。采用实时荧光定量PCR检测大菱鲆和红鳍东方鲀幼鱼不同组织中以及不同营养状态下FATP mRNA的相对表达量。结果表明:1)大菱鲆和红鳍东方鲀FATP与其他鱼类和哺乳动物中的同源序列具有高度一致性,但FATP6与FATP1和FATP4的同源性较低。2)大菱鲆FATP1在大脑、心脏、眼睛和肾脏中高表达,红鳍东方鲀FATP1在肝脏中高表达。大菱鲆和红鳍东方鲀FATP4在消化道中高表达。大菱鲆FATP6在幽门盲肠和前肠中高表达,而红鳍东方鲀FATP6仅在肝脏中高表达。3)饲料脂肪水平对大菱鲆和红鳍东方鲀的肝脏FATP1、FATP4和FATP6的mRNA相对表达量无显著影响(P>0.05)。4)大菱鲆第30天的肝脏FATP1的mRNA相对表达量显著高于第0天、第10天和第20天(P<0.05)。红鳍东方鲀第16天和第31天的肝脏FATP1的mRNA相对表达量显著高于第0天、第1天、第4天和第9天(P<0.05),第9天和第31天的肝脏FATP4的mRNA相对表达量显著高于第0天、第1天和第4天(P<0.05),第16天的肝脏FATP6的mRNA相对表达量显著高于第0天、第1天、第4天、第9天和第31天(P<0.05)。由此可见,大菱鲆和红鳍东方鲀FATP表达的组织分布模式不同,但FATP表达对饲料脂肪水平和饥饿时间的响应相似。饲料脂肪水平对大菱鲆和红鳍东方鲀肝脏FATP1、FATP4和FATP6的表达均无显著影响,但饥饿可上调红鳍东方鲀肝脏FATP1、FATP4和FATP6以及大菱鲆肝脏FATP1的表达。In the present study,three fatty acid transport protein(FATP)isoforms,namely FATP1,FATP4 and FATP6 in two marine teleosts(Scophthalmus maximus and Takifugu rubripes)were characterized in terms of sequence analysis,as well as the gene expression characteristics in different tissues and different nutritional status were studied.In the study of tissue distribution of FATP expression,the different tissues of Scophthalmus maximus and Takifugu rubripes were selected.In the feeding experiment,juvenile fish of Scophthalmus maximus and Takifugu rubripes were fed experimental diets with different lipid levels(8%,12%and 16%)for 9 weeks,followed by a starvation experiment(30 d).The FATP mRNA relative expression levels of in different tissues and different nutritional status of Scophthalmus maximus and Takifugu rubripes were detected by real-time fluorescent quantitative PCR.The results showed as follows:1)the FATP of Scophthalmus maximus and Takifugu rubripes showed high identity to their orthologs in other fish species and mammals,but FATP6 showed a low identity to FATP1 and FATP4.2)The FATP1 of Scophthalmus maximus was high expression in brain,heart,eye and kidney,and the FATP1 of Takifugu rubripes was high expression in liver.The FATP4 of Scophthalmus maximus and Takifugu rubripes was high expression in digestive tract.The FATP6 of Scophthalmus maximus was high expression in pyloric caecum and anterior intestine,and the e FATP6 of Takifugu rubripes was only high expression in liver.3)Dietary lipid levels had no significant effects on mRNA relative expression levels of FATP1,FATP4 and FATP6 in liver of Scophthalmus maximus and Takifugu rubripes(P>0.05).4)The liver FATP1 mRNA relative expression level of Scophthalmus maximus on day 30 was significantly higher than that on day 0,day 10 and day 20(P<0.05).The liver FATP1 mRNA relative expression level of Takifugu rubripes on day 16 and day 31 was significantly higher than that on day 0,day 1,day 4 and day 20(P<0.05),the liver FATP4 mRNA relative expression level on day 9 and

关 键 词：大菱鲆 红鳍东方鲀 基因表达 饲料脂肪水平 饥饿 

分 类 号：S917[农业科学—水产科学]