火棘果原花青素的提取优化与抗氧化活性研究  

Optimization of Extraction Process and Antioxidant Activity of Proan⁃thocyanidins from Pyracantha fortuneana Fruit

作　　者：张星和 杨晓娜 邹章玉 左荣艳 ZHANG Xinghe;YANG Xiaona;ZOU Zhangyu;ZUO Rongyan(School of Resources and Environment,Baoshan University;Key Laboratory of High-value Conversion and Utilization of Biomass Resources in Nujiang River Valley for Colleges and Universities of Yunnan Province,Baoshan Yunnan 678000,China)

机构地区：[1]保山学院资源环境学院 [2]云南省高校怒江河谷生物质资源高值转化与利用重点实验室,云南保山678000

出　　处：《保山学院学报》2022年第5期19-27,共9页JOURNAL OF BAOSHAN UNIVERSITY

基　　金：云南省科技厅地方高校联合青年项目“基于板栗壳开发高效、经济的饮用水中重金属汞深度净化材料研究”(项目编号:202001BA070001-110);国家级大学生创新训练计划项目“保山荔枝中功能性成分的提取及其体外降血脂活性的研究”(项目编号:202010686002)。

摘　　要：在单因素、正交试验基础上,确定火棘果原花青素的最佳提取条件为乙醇浓度85%、水料比40 mL/g、微波功率560 W和微波时间80 s,该工艺条件下原花青素的提取率为12.51 mg/g;通过OH·清除率、DPPH·清除率、ABTS^(+)·清除率、金属离子螯合能力、总抗氧化性以及还原能力共6个抗氧化方法对提取的原花青素的抗氧化活性进行了综合评价;结果显示:除金属离子螯合能力较弱外,其余5种抗氧化活性结果均证明火棘果原花青素具有抗氧化能力,且对OH·、DPPH·、ABTS^(+)·的清除能力较强,其中对ABTS^(+)清除率达到94%。On the basis of single factor experiments and orthogonal design of four factors and three levels of experiments,the optimizing extraction process of proanthocyandins from pyracantha fortuneana fruits was:ethanol concentration 85%,water-to-material ratio 40 mL/g,microwave power 560 W,and microwave time 80 s.Under these conditions,the extraction rate of proanthocyanidins was 12.51 mg/g.The antioxidant activity by microwave-assisted extraction was comparatively evaluated by radical scavenging activities against OH·,DPPH·,ABTS^(+),ferrousion chelating activity,total antioxidant capacity and reducing power.The results showed that 5 antioxidant assays consistently indicated the antioxidant capacity of proanthocyandins extracted from Pyracantha fortuneana fruit except for ferrous ion chelation.Moreover,the extracted proanthocyandins showed the strongest ability to scavenge free radicals.

关 键 词：火棘果 原花青素 微波辅助法 抗氧化 

分 类 号：Q1[生物学—普通生物学]