亚麻叶白千层内生真菌Penicillium citrinum YMY-1次生代谢产物研究  

Secondary metabolites of endophytic fungus Penicillium citrinum YMY-1 from Melaleuca linariifolia

作　　者：张莎 邓小华[3] 旷敏 彭伟伟 郑雨婷 许立 曾广源 艾卫敏 邹振兴 ZHANG Sha;DENG Xiao-hua;KUANG Min;PENG Wei-wei;ZHENG Yu-ting;XU Li;ZENG Guang-yuan;AI Wei-min;ZOU Zhen-xing(Xiangya School of Pharmaceutical Sciences,Central South University,Changsha 410013;Hunan Key Laboratory of Diagnostic and Therapeutic Drugs for Chronic Diseases,Changsha 410013;Department of Human Anatomy and Neurobiology,School of Basic Medical Science,Central South University,Changsha 410013;Department of Human Anatomy and Physiology,Section of Basic Medical Science,Xiangtan Medical and Health Vocational and Technical College,Xiangtan Hunan 411012)

机构地区：[1]中南大学湘雅药学院,长沙410013 [2]慢病诊疗小分子药物发现与转化湖南省重点实验室,长沙410013 [3]中南大学基础医学院人体解剖学与神经生物学系,长沙410013 [4]湘潭医卫职业技术学院医学基础课部人体解剖学与生理学教研室,湖南湘潭411012

出　　处：《中南药学》2022年第10期2223-2227,共5页Central South Pharmacy

基　　金：湖南省自然科学基金(No.2021JJ30917);湖南省高新技术产业科技创新引领计划(No.2020GK4083);河南省“揭榜挂帅”科技项目(No.211110110300);海南省重点研发项目(No.ZDYF2022SHFZ048);湖南省科教联合基金(No.2019JJ70043)。

摘　　要：目的 研究亚麻叶白千层植物内生真菌Penicillium citrinum YMY-1的化学成分。方法 采用大孔树脂、硅胶、ODS、Sephadex-LH-20柱色谱及Pre-HPLC现代色谱方法进行分离纯化,并通过HR-ESI-MS、NMR等波谱手段对所得化合物进行结构鉴定。结果 从中分离出12个化合物,分别鉴定为chromosulfine(1)、methyl 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate(2)、penicitrinone A(3)、rubasperone B(4)、quinolactacin Al(5)、quinolactacin A2(6)、3,4,5-三甲基-1,2-苯二酚(7)、12-hydroxysorbicillin(8)、2-(hept-5-enyl)-3-methyl-4-oxo-6,7,8,8a-tetrahydro-4H-pyrrolo[2,1-b]-1,3-oxazine(9)、dihydrocitrinone(10)、6,8-dihydroxy-3,4,5,7-tetramethyl-3,4-dihydroisocoumarin(11)和β-麦角甾醇(12)。结论 化合物4和8为首次从青霉属真菌中分离得到。Objective To determine the chemical constituents of endophytic fungus Penicillium citrinum YMY-1 from Melaleuca linariifolia.Methods The fermentation of Penicillium citrinum YMY-1 was isolated and purified with macroporous resin,silica gel,ODS,Sephadex-LH-20 column chromatography as well as pre-HPLC,and the structures of obtained compounds were identified by HR-ESI-MS and NMR.Results Totally 12 compounds were isolated and identified as chromosulfine (1),methyl 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (2),penicitrinone A (3),rubasperone B (4),quinolactacin Al (5),quinolactacin A2 (6),3,4,5-trimethyl-1,2-benzenediol (7),12-hydroxysorbicillin (8),2-(hept-5-enyl)-3-methyl-4-oxo-6,7,8,8a-tetrahydro-4H-pyrrolo[2,1-b]-1,3-oxazine (9),dihydrocitrinone (10),6,8-dihydroxy-3,4,5,7-tetramethyl-3,4-dihydroisocoumarin (11),and β-ergosterol (12).Conclusion Compounds 4 and 8 have been isolated from the genus of Penicillium for the first time.

关 键 词：内生真菌 亚麻叶白千层 青霉属 Penicillium citrinum YMY-1 化学成分 

分 类 号：R284.1[医药卫生—中药学]