植物根际土壤真菌Trichoderma velutinum的化学成分研究  

Chemical constituents of plant rhizosphere soil fungus Trichoderma velutinum

作　　者：旷敏 彭伟伟 许立 郑雨婷 桑子焕 秦思雨 邹振兴 KUANG Min;PENG Wei-wei;XU Li;ZHENG Yu-ting;SANG Zi-huan;QIN Si-yu;ZOU Zhen-xing(Xiangya School of Pharmaceutical Sciences,Central South University,Changsha 410013;Hunan Key Laboratory of Diagnostic and Therapeutic Drugs for Chronic Diseases,Central South University,Changsha 410013)

机构地区：[1]中南大学湘雅药学院,长沙410013 [2]慢病诊疗小分子药物发现与转化湖南省重点实验室,长沙410013

出　　处：《中南药学》2022年第9期1975-1981,共7页Central South Pharmacy

基　　金：湖南省自然科学基金项目(No.2021JJ30917);湖南省高新技术产业科技创新引领计划(No.2020GK4083);河南省“揭榜挂帅”科技项目(No.211110110300);海南省重点研发项目(No.ZDYF2022SHFZ048);湖南省科教联合基金项目(No.2019JJ70043)。

摘　　要：目的 对根际土壤真菌毛绒木霉Trichoderma velutinum次级代谢产物进行研究。方法 利用大孔树脂、正相硅胶、ODS、Sephadex LH-20和Pre-HPLC等现代色谱技术对菌株毛绒木霉的固体发酵物乙酸乙酯提取部位化学成分进行分离纯化;利用NMR、HR-ESI-MS等波谱学技术对化合物的结构进行表征。结果 从Trichoderma velutinum中共分离鉴定了13个化合物:chaetoglobosin E(1)、chaetoglobosin Fex(2)、armochaetoglobin S(3)、7-O-acetylarmochaetoglobin S(4)、cyclo-(L-Trp-D-Ile)(5)、cyclo-(L-Trp-L-Leu)(6)、cyclo-(L-Trp-L-Phe)(7)、cyclo-(L-Trp-L-Trp)(8)、cyclo-(L-Ile-L-Phe)(9)、cyclo-(L-Leu-L-Phe)(10)、2’-O-methyladenosine(11)、β-adenosine(12)、N-acetyltryptamine(13)。结论 化合物1~10为首次从木霉属真菌中分离得到。Objective To determine the secondary metabolites of the rhizosphere soil fungus Trichoderma velutinum.Methods The chemical constituents of ethyl acetate extraction of the solid fermentation product of Trichoderma velutinum were separated and purified by modern chromatographic techniques such as macroporous resin,silica gel,ODS,Sephadex LH-20 and Pre-HPLC.The structures of the compounds were characterized by spectroscopic techniques such as NMR and HR-ESI-MS.Results Totally 13 compounds were isolated and identified as chaetoglobosin E (1),chaetoglobosin Fex (2),armochaetoglobin S (3),7-O-acetylarmochaetoglobin S (4),cyclo-(L-Trp-D-Ile) (5),cyclo-(L-Trp-L-Leu) (6),cyclo-(L-Trp-L-Phe) (7),cyclo-(L-Trp-L-Trp) (8),cyclo-(L-Ile-L-Phe) (9),cyclo-(L-Leu-L-Phe) (10),2’-O-methyladenosine (11),β-adenosine (12),and N-acetyltryptamine (13).Conclusion Compounds 1~10 have been isolated from Trichoderma for the first time.

关 键 词：根际土壤真菌 毛绒木霉 化学成分 细胞松弛素 二酮哌嗪 

分 类 号：R284.1[医药卫生—中药学]