基于中国知网文献计量分析脓毒症小鼠模型的研究  

Research status of sepsis mouse models based on bibliometric analysis of CNKI literatures

作　　者：田圆 梁群[2] 潘郭海容 张典 贾璇 吴丽丽[4] Tian Yuan;Liang Qun;Pan Guo-hai-rong;Zhang Dian;Jia Xuan;Wu Li-li(Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第一医院急诊及重症医学科,黑龙江哈尔滨150040 [3]复旦大学附属华山医院,上海200400 [4]广州中医药大学深圳医院,广东深圳518048

出　　处：《中国急救医学》2022年第11期982-988,共7页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金面上项目(81974557);国家自然科学基金面上项目(81774276);国家中医管理局新冠肺炎中医药应急专项课题(2020ZYLCYJ 06-2);黑龙江中医药大学校级科技创新研究平台项目(2018pt06)。

摘　　要：目的对近年国内高水平脓毒症小鼠实验研究进行文献计量分析,了解其应用状况、发展趋势及存在的问题,对推进脓毒症临床前期研究提供参考。方法对2019年1月至2022年4月期间国内中国知网(CNKI)双核心期刊(即北大中文核心、中国科学引文数据库和中国科技核心期刊同时具备其中任意二者)中脓毒症方向运用小鼠的研究进行整理计量,分析目前国内脓毒症小鼠模型的品系应用、性别分布、机制方向及造模手段等现状。结果共得到来自53家期刊的126篇运用脓毒症小鼠模型的实验研究,其中脓毒症脑损伤为近期的研究热点(34篇,26.98%),新兴的研究机制以细胞焦亡、肠道菌群及铁死亡为主。研究中所用小鼠品系以C57亚系为主(89/126),C57BL/6最多(54/89);研究多采用单雄性小鼠设计(110,87.30%);造模方式以盲肠结扎手术(CLP)法为主(84,67.2%),但各研究盲肠结扎位置。穿刺次数和针头型号存在较大差异;其次为脂多糖(LPS)注射法(39,31.2%)。结论目前国内脓毒症小鼠实验研究中小鼠的品系背景、性别构成、所处年龄和造模手段均存在较大差异,且存在国内研究人员普遍不够重视,报道常有缺失,造成研究结果复现能力不佳、难以推广转化等问题。急需对脓毒症模型造模手段的标准化展开相应研究,并推出成体系的模型报告标准,这是研究可重复性和可信度的基础保证。Objective To conduct a bibliometric analysis of high-level experimental studies on septic mice in recent years in China,and to understand their application status,development trends and existing problems so as to provide a reference and promotion for preclinical studies on sepsis.Methods From January 2019 to April 2022,the researches on sepsis using mice in the"double core"journal of CNKI(Peking University Chinese core,China Science Citation Database,and China Science and Technology core journals)were collated and calculated,and the current situation of domestic sepsis mouse models were analyzed in dimensions including strain application,gender distribution,mechanism direction,and modeling means.Results A total of 126 experimental studies using sepsis mouse models were obtained from 53 journals,among which sepsis brain injury was the recent research hotspot(34 articles,26.98%),and the emerging research mechanisms were mainly cell death,intestinal flora and iron death.C57 subline was the main mouse strain used in the studies(89/126),in which C57BL/6 was the most(54/89);Single male mice were mostly used in the studies(110,87.30%);Cecal ligation was the main method of modeling(84,67.2%),but there were significant differences in the location of cecal ligation,the number of punctures and the types of needle;Lipopolysaccharide injection was the second most method(39,31.2%).Conclusions At present,there are significant differences between the experimental studies of septic mice in China in terms of strain background,gender composition,age and modeling methods.Domestic researchers generally do not pay enough attention to it,and the reports are often missing,resulting in poor reproducibility of research results and difficulty in promotion and transformation.There is an urgent need to carry out corresponding research on the standardization of modeling methods for sepsis models,and to develop a systematic standard form of model reports,which is the basic guarantee for the repeatability and reliability of research.

关 键 词：脓毒症 文献计量 小鼠模型 小鼠品系 中国知网(CNKI) 

分 类 号：R459.7[医药卫生—急诊医学] R-332[医药卫生—治疗学]