基于P38 MAPK信号通路探讨扶正利湿抗癌方含药血清对前列腺癌LNCaP细胞增殖及凋亡的影响  

Discussion on Effects of Serum Containing Fuzheng Lishi Kang’ai Decoction(扶正利湿抗癌方)on Proliferation and Apoptosis of Prostate Cancer LNCaP Cells based on P38/MAPK Signaling Pathway

作　　者：王鹏 乔泽昀 赵文兵 WANG Peng;QIAO Zeyun;ZHAO Wenbing(Shanxi Hospital of Integrated Chinese and Western Medicine,Taiyuan,Shanxi 030013;Shanxi University of Chinese Medicine,Jinzhong,Shanxi 030619)

机构地区：[1]山西省中西医结合医院,山西太原030013 [2]山西中医药大学,山西晋中030619

出　　处：《中国中医药科技》2022年第6期963-967,共5页Chinese Journal of Traditional Medical Science and Technology

基　　金：山西省自然科学基金(201901D111401)。

摘　　要：目的:研究扶正利湿抗癌方含药血清对LNCaP细胞增殖、凋亡及p38 MAPK通路的作用。方法:应用CCK-8法观察扶正利湿抗癌方含药血清对LNCaP细胞的增殖抑制作用,以流式细胞仪分析扶正利湿抗癌方含药血清对LNCaP细胞凋亡的影响,Western Blot法检测LNCaP细胞p38 MAPK、p-p38 MAPK、Bax、Bcl-2、Caspase-3、p53等蛋白的表达。结果:扶正利湿抗癌方含药血清能够以剂量依赖性地抑制LNCaP细胞增殖并诱导其凋亡,上调p-p38 MAPK、Bax、Caspase-3及p53蛋白表达,下调Bcl-2蛋白表达水平。结论:扶正利湿抗癌方含药血清可能通过激活p38 MAPK信号传导通路抑制LNCaP细胞增殖并促进其凋亡。Objective:To study the effects of serum containing Fuzheng Lishi Kang’ai Decociton on the proliferation,apoptosis and p38 MAPK pathway of LNCaP cells.Methods:CCK-8 method was used to observe the inhibitory effect of serum containing Fuzheng Lishi Kang’ai Decociton on the proliferation of LNCaP cells.The effect of serum containing Fuzheng Lishi Kang’ai Decociton on the apoptosis of LNCaP cells was analyzed by flow cytometry.The Expressions of p38 MAPK,p-p38 MAPK and Bax,Bcl-2,caspase-3 and p53 protein were detected by Western blot.Results:The serum containing Fuzheng Lishi Kang’ai Decociton could inhibit the proliferation of LNCaP cells and induce apoptosis in a dose-dependent manner,up regulate the expressions of p-p38 MAPK,Bax,caspase-3 and p53 protein,and down regulate the expression of Bcl-2 protein.Conclusion:The serum containing Fuzheng Lishi Kang’ai Decociton can inhibit the proliferation and promote the apoptosis of LNCaP cells by activating p38 MAPK signal transduction pathway.

关 键 词：前列腺癌 扶正利湿抗癌方 血清药理学 p38 MAPK通路 增殖 凋亡 体外实验 

分 类 号：R285[医药卫生—中药学]