复方甘菊利多卡因凝胶对大鼠皮肤创面愈合的影响  

Effect of compound chamomile and lidocaine hydrochloride gel on skin wound healing in rats

作　　者：谢振年[1] 安晓静 周海洋 王芳丽[4] 王立平 XIE Zhennian;AN Xiaojing;ZHOU Haiyang;WANG Fangli;WANG Liping(Department of Anorectal Surgery,Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing,100091;Department of Pathology,Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing,100091;Department of General Surgery,Air Force Medical Center of People′s Liberation Army,Beijing,100142;Department of General Surgery,Aerospace Center Hospital,Beijing,100049;Beijing Fengyi Anorectal Hospital,Beijing,100000)

机构地区：[1]中国中医科学院西苑医院肛肠外科,北京100091 [2]中国中医科学院西苑医院病理科,北京100091 [3]中国人民解放军空军特色医学中心普外科,北京100142 [4]航天中心医院普外科,北京100049 [5]北京丰益肛肠医院,北京100000

出　　处：《实用临床医药杂志》2022年第17期18-23,共6页Journal of Clinical Medicine in Practice

基　　金：北京肛肠学会科研基金(ABCP2021-001);中国中医科学院科技创新工程(C2021A01909)。

摘　　要：目的探讨复方甘菊利多卡因凝胶对大鼠皮肤创面愈合的影响及作用机制。方法采用手术切除的方法建立皮肤创面大鼠动物模型,并随机分为实验组(复方甘菊利多卡因凝胶)、阳性药组(复方多黏菌素B软膏)和模型组(生理盐水)。治疗7、14 d时,测量各组大鼠创面大小。采用酶联免疫吸附测定(ELISA)法检测创面组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-1β(IL-1β)水平。采用免疫组化检测创面组织中CD68、CD163和CC趋化因子受体7(CCR7)的表达,观察巨噬细胞表型的变化。采用苦味酸-天狼猩红染色观察创面组织中胶原的分布。结果治疗7 d时,实验组炎症细胞、血管减少,成纤维细胞增加,较阳性药组更具优势。治疗14 d时,实验组创面以成纤维细胞和胶原为主,与阳性药组比较,差异无统计学意义(P>0.05)。治疗7 d时,实验组大鼠创面组织中TNF-α、IL-6和IL-1β的表达水平均低于模型组、阳性药组,差异有统计学意义(P<0.05或P<0.01)。治疗14 d时,各组大鼠创面组织中TNF-α、IL-6和IL-1β的表达差异无统计学意义(P>0.05)。治疗7、14 d时,实验组中胶原含量显著增加,但巨噬细胞的表型无明显变化。结论复方甘菊利多卡因凝胶对皮肤创面具有较好的抗炎和促进愈合的作用,创伤早期抑制炎症因子释放可能是其主要作用机制之一。Objective To explore the effect and mechanism of compound chamomile and lidocaine hydrochloride gel on skin wound healing in rats.Methods The model rats with skin wound was established by surgical resection,and the rats were randomly divided into experimental group(treated with compound chamomile and lidocaine hydrochloride gel),positive drug group(treated with compound polymyxin B ointment) and model group(treated with normal saline).At 7 and 14 days of treatment,the wound size of rats in each group was measured.Tumor necrosis factor-α(TNF-α),interleukin-6(IL-6) and interleukin-1β(IL-1β) levels in wound tissues were detected by enzyme-linked immunosorbent assay(ELISA).The expression levels of CD68,CD163 and CC chemokine receptor 7(CCR7) in the wound tissues were detected by immunohistochemistry,and the change of macrophage phenotype was observed.The distribution of collagen in wound tissues was observed by Picric acid Sirius red staining.Results After 7 days of treatment,the inflammatory cells and blood vessels decreased while the fibroblasts increased in the experimental group,which was more advantageous than the positive drug group.At the 14 th day of treatment,the wound healing of the experimental group was mainly composed of fibroblasts and collagen,and there was no significant difference between the experimental group and the positive drug group( P > 0.05).At 7 days of treatment,the expression levels of TNF-α,IL-6 and IL-1β in the experimental group were significantly lower than those of model group and positive drug group( P 0.05).At 7 and 14 days of treatment,the collagen content in the experimental group increased significantly,but the phenotype of macrophages did not change obviously.Conclusion Compound chamomile and lidocaine hydrochloride gel has good anti-inflammatory and healing effects on skin wounds,and inhibiting the release of inflammatory factors may be one of its main mechanisms.

关 键 词：皮肤创面 复方甘菊利多卡因凝胶 大鼠 炎症因子 成纤维细胞 巨噬细胞 

分 类 号：R961[医药卫生—药理学] R622[医药卫生—药学]