边疆高原地区彝族人群rs1333049位点单核苷酸多态性与冠心病易感性的关系  

Correlation between single nucleotide polymorphism rs1333049 and susceptibility to coronary heart disease of Yi ethnic people in frontier plateau area

作　　者：刘斌[1] 张敏[1] 李显刚[1] 杨志敏[1] 周芳[1] 黄陈斌 陈洋宝 杨德梅 LIU Bin;ZHANG Min;LI Xiangang;YANG Zhimin;ZHOU Fang;HUANG Chenbin;CHEN Yangbao;YANG Demei(Department of Cardiology,the Fifth Affiliated Hospital of Kunming Medical University,Diannan Central Hospital of Honghe Prefecture,Gejiu,Yunnan,661000)

机构地区：[1]昆明医科大学第五附属医院/红河州滇南中心医院心血管内科,云南个旧661000

出　　处：《实用临床医药杂志》2022年第17期24-28,36,共6页Journal of Clinical Medicine in Practice

基　　金：云南省教育厅科学研究基金项目(2018JS257,2020J0236)。

摘　　要：目的 探讨边疆高原地区世居彝族人群rs1333049位点单核苷酸多态性(SNP)与冠心病易感性的关系。方法 采用随机数表法选取在红河州滇南中心医院住院的边疆高原地区世居彝族患者120例作为研究对象,根据临床诊断结果分为冠心病组和对照组,每组60例。采集患者外周静脉血,采用TaqMan实时荧光定量聚合酶链反应(PCR)检测染色体9p21.3区域rs1333049位点基因分型。比较冠心病组和对照组的基因型(CC、CG、GG)频率、等位基因(C、G)频率和基因量,分析不同疾病阶段冠心病患者rs1333049基因型频率、等位基因频率和基因量,采用多因素Logistic回归分析探讨冠心病患者rs1333049 CG基因表达的影响因素。结果 冠心病组rs1333049 SNP位点CG基因型频率、CG基因量均高于对照组,差异有统计学意义(P<0.05);冠心病组中的急性心肌梗死患者rs1333049 SNP位点CG基因型频率、CG基因量均高于对照组,差异有统计学意义(P<0.05);冠心病组中的不稳定性心绞痛患者、稳定性心绞痛患者rs1333049 SNP位点的基因型频率、等位基因频率及基因量与对照组比较,差异均无统计学意义(P>0.05)。边疆高原地区世居彝族冠心病患者的rs1333049位点CG基因量表达与左主干(LM)/左前降支(LAD)病变有关(P<0.05),与血脂指标、空腹血糖(FBG)、冠心病不同疾病阶段、右冠状动脉病变、左回旋支病变及各支冠状动脉狭窄程度(Gensini积分)无关(P>0.05)。多因素Logistic回归分析显示,FBG(OR=1.55,95%CI为1.01~2.36)、LM/LAD病变(OR=1.87,95%CI为1.10~3.16)是边疆高原地区彝族冠心病患者rs1333049 CG基因表达的独立影响因素(P<0.05)。结论 rs1333049 CG基因为边疆高原地区世居彝族人群的冠心病易感基因,而FBG水平、LM/LAD病变是rs1333049 CG基因表达的影响因素。Objective To explore the relationship between single nucleotide polymorphism(SNP) rs1333049 and susceptibility to coronary heart disease of permanently inhabited Yi ethnic people in frontier plateau area.Methods Using the random number table method,120 cases of permanently inhabited Yi ethnic patients of frontier plateau area in Diannan Central Hospital of Honghe Prefecture were selected as study objects,and were divided into coronary artery disease group and control group according to clinical diagnosis results,with 60 cases in each group.Peripheral venous blood was collected and rs1333049 genotype in 9 p21.3 region of chromosomewas detected by TaqMan real-time fluorescence quantitative polymerase chain reaction(PCR).The frequency of genotypes(CC,CG,GG),frequency of allele(C,G) and gene amount were compared between the coronary artery disease group and the control group.The genotype frequency,allele frequency and gene amount of rs1333049 in coronary artery disease patients with different disease stages were analyzed.MultivariateLogistic regression analysis was used to explore the influencing factors of rs1333049 CG gene expression in patients with coronary heart disease.Results The CG genotype frequency and CG gene amount of rs1333049 SNP locus in the coronary artery disease group were higher than those in the control group( P 0.05).CG gene amount expression of rs1333049 was associated with left main branch( LM)/left anterior descending branch( LAD) lesions in permanently inhabited Yi ethnic patients with coronary artery disease in the border plateau area( P 0.05).Multivariate Logistic regression analysis showed that FBG( OR =1.55;95% CI,1.01 to 2.36),LM/LAD lesions( OR = 1.87;95% CI,1.10 to 3.16) were independent influencing factors of rs1333049 CG gene expression in Yi ethnic patients with coronary heart disease in border and plateau areas( P < 0.05).Conclusion The rs1333049 CG gene is a susceptibility gene for coronary heart disease in permanently inhabited Yi ethnic people in frontier plateau,while FBG and L

关 键 词：彝族 基因多态性 冠心病 影响因素 高原地区 易感基因 

分 类 号：R541.4[医药卫生—心血管疾病] R4[医药卫生—内科学]