茶多酚功能化的还原氧化石墨烯的制备和生物性能  

Preparation and biological properties of TPG

作　　者：郝丽英 罗萌 康茂萍 HAO Liying;LUO Meng;KANG Maoping(State Key Laboratory of Oral Diseases,West China Hospital of Stomatology,Sichuan University,Chengdu 610064,China;Department of Energy Chemistry and Materials Engineering,Shanxi Institute of Energy,Jinzhong 030600,China)

机构地区：[1]四川大学华西口腔医学院口腔疾病研究重点实验室,四川成都610064 [2]山西能源学院能源化学与材料工程系,山西晋中030600

出　　处：《实验技术与管理》2022年第10期49-53,114,共6页Experimental Technology and Management

基　　金：四川省科技计划项目(2020YFS0073);大学生创新创业训练计划(20221276L)。

摘　　要：针对当前纳米药物构建的研究热点,以改性石墨烯为载药材料,研究材料生物性能和纳米药物的释放。采用一步绿色还原和功能化法合成茶多酚功能化的还原氧化石墨烯(TPG);通过稳定性和细胞毒性实验,探讨TPG的生物性能;以TPG为载体材料,盐酸阿霉素(DOX)为负载化疗药物,从形貌、结构上验证纳米药物的成功合成;通过调节pH,研究不同pH对纳米药物释放的影响。结果表明:TPG具有良好的生物相容性,可有效负载DOX获得纳米药物,负载容量高达8.299×10^(4) mg/g,具有pH依赖的释放行为和荧光开关性能,在pH为5.0时具有更高的药物释放效率。该实验为缓释药物的开发和癌症的诊疗和实时监测提供前期研究基础和指导意义。In view of the current research focus on the construction of nanodrugs,the biological properties of the modified graphene as the drug delivery material and the release of nanodrugs are investigated.Tea polyphenol functionalized and reduced graphene oxide(TPG)is synthesized by one-step green reduction and functionalization.The biological properties of TPG are investigated by stability and cytotoxicity tests.The nanodrugs based on TPG as the carrier material and DOX as the loaded chemotherapy drug are successfully synthesized and verified from the morphology and structure.The effect of different pH on the release of nanodrugs is studied.The results show that TPG has good biocompatibility,and high loading capacity of DOX,which is up to 8.299×10^(4) mg/g,and has pH-dependent release behavior and fluorescence switching performance,showing higher drug release efficiency at pH 5.0.This experiment provides a preliminary research basis and guiding significance for the development of sustained-release drugs and the diagnosis,treatment,and real-time monitoring of cancer.

关 键 词：茶多酚功能化的还原氧化石墨烯(TPG) 生物性能 负载容量 纳米药物 

分 类 号：TB34[一般工业技术—材料科学与工程]