东方蝾螈脊髓损伤模型的建立及其评价  

Establishment of Spinal Cord Injury Model and Behavioral Score in cynops orientalis

作　　者：王迪 赵曼 陈杰 汤枭 李君[1] WANG Di;ZHAO Man;CHEN Jie;TANG Xiao;LI Jun(School of Life Science,Anhui Normal University,Wuhu,Anhui 241000;Wuhu No.2 People’s Hospital,Wuhu,Anhui 241000)

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]芜湖第二人民医院,安徽芜湖241000

出　　处：《绍兴文理学院学报》2022年第10期41-46,共6页Journal of Shaoxing University

基　　金：国家自然科学基金项目“Ufm1泛素化样修饰系统E3连接酶—Ufl1缺失调控动物肝脏发育和原发性肝纤维化发生的分子机制”(31970413);安徽省自然科学基金项目“RB1CC1基因对小鼠次级脊髓损伤调节的分子机制及修复研究”(1908085MC83);芜湖市科技计划项目“ZBTB38/FIP200自噬信号通路对次级脊髓损伤调节的分子机制”(2019cg19).

摘　　要：与哺乳动物相比,东方蝾螈具有较强的脊髓组织再生能力.成功构建了东方蝾螈脊髓损伤的模型,对其再生过程进行行为学评估,确定蝾螈脊髓损伤手术后受损的脊髓可以再生,为神经再生的研究提供基础.实验使用10月龄的东方蝾螈,剥离脊椎骨,对脊髓进行直接横断,观察其恢复过程并对第0、2、4、7、14、21天的后肢运动和游泳情况进行行为学评分,并根据评分结果对其修复过程4、10天的组织进行染色观察.结果表明:东方蝾螈经脊髓横断后后肢虽失去运动功能,但短期内其受损脊髓可以自主恢复.第7天开始出现运动和游泳功能恢复,第10天部分细胞呈现规则排列,三周左右基本恢复行动力.本研究将为研究脊髓损伤再生相关的细胞和分子通路提供重要的动物模型,为实现哺乳动物脊髓损伤的修复提供必要的实验基础.Compared with mammals,Cynops orientalis is more capable of spinal cord tissue regeneration.In this paper,the model of spinal cord injury of Cynops orientalis was successfully constructed,and the behavioral evaluation of its regeneration process was conducted to determine that the injured spinal cord of salamander spinal cord injury can be regenerated after operation,thus providing a basis for the study of nerve regeneration.In the experiment,a 10-month-old Cynops orientalis was used to peel off the vertebrae and transect the spinal cord directly.The recovery process was observed and the movement and swimming of hindlimbs on the 0,2,4,7,14 and 21 days were scored.According to the score results,the tissues of the repair process for 4 and 10 days were stained and observed.The results show that although the hindlimb of Cynops orientalis loses motor function after spinal cord transection,the injured spinal cord can recover spontaneously in a short time.The function of exercise and swimming begins to recover on the 7th day,and some cells show regular arrangement on the 10th day,and basically recover in about three weeks.This study provides an important animal model for the study of cellular and molecular pathways related to spinal cord injury and regeneration,hence a necessary experimental basis for the repair of spinal cord injury in mammals.

关 键 词：东方蝾螈 脊髓损伤 再生 模式生物 行为学 

分 类 号：Q331[生物学—遗传学]