长链非编码RNA-Hexokinase 2的生物信息学分析  

Bioinformatics Analysis of Long Non-coding RNA-Hexokinase 2

作　　者：魏晨 蒋振[1] WEI Chen;JIANG Zhen(Department of Biochemistry and Molecular Biology,School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院基础医学与法医学院生物化学与分子生物学教研室,四川南充637000

出　　处：《医学信息》2022年第19期1-5,10,共6页Journal of Medical Information

基　　金：南充市科技局项目(编号:20SXJCQN0004、18SXHZ0281)。

摘　　要：目的运用生物信息学分析方法,分析长链非编码RNA-Hexokinase 2(HK2)在细胞内的调控靶基因及信号通路,并对其进行生物学功能注释,阐述lncRNA-HK2在细胞内的功能作用。方法在miRDB数据库和Starbase3.0数据库中预测与lncRNA-HK2有miRNA-lncRNA互作关系的miRNA,将结果取交集。在Starbase3.0数据库中预测miRNAs靶基因,并进行GO功能注释和Pathway通路富集分析。结果在miRDB数据库预测到50个miRNAs与lncRNA-HK2相作用,Starbase3.0数据库预测到14个miRNAs与之相作用,取2个数据库的交集得到hsa-miR-374a-5p、hsa-miR-374b-5p 2个miRNAs;GO功能注释显示hsa-miR-374a-5p的靶基因主要调控蛋白磷酸化、肿瘤发生通路、转录调控、DNA损伤反应、脂肪细胞分化及胚胎发育等基因及信号通路;hsa-miR-374b-5p作用的靶基因主要调控基因表达,调控序列特异性DNA结合转录因子的活性、翻译调控、细胞对于胰岛素的反应、细胞粘附及细胞周期等生物学过程,信号通路富集结果主要分布在TNF信号通路、RAS信号通路、人乳头状瘤病毒感染、转录错误调控及多种肿瘤信号通路等方面。结论长链非编码RNA HK2在细胞内的重要的调控作用,具体与蛋白磷酸化、肿瘤发生通路、转录调控、DNA损伤反应、翻译以及细胞周期等多条信号通路密切相关,其主要通过上述作用通路调控生理和病理进程。Objective To analyze the regulatory target genes and signaling pathways of lncRNA-HK2 in cells by bioinformatics analysis,and annotate its biological functions,so as to explain the functions of lncRNA-HK2 in cells.Methods The miRNAs interacting with lncRNA-HK2 were predicted in miRDB database and Starbase3.0 database,and the results were intersected.Target genes of miRNAs were predicted in the Starbase 3.0 database,and GO functional annotation and Pathway enrichment analysis were performed.Results A total of 50 miRNAs were predicted to interact with lncRNA-HK2 in miRDB database,and 14 miRNAs were predicted to interact with lncRNA-HK2 in Starbase3.0 database.A total of 2 miRNAs from the intersection of the two databases were selected for further analysis.GO functional annotation showed that the target genes of hsa-miR-374a-5p mainly regulated protein phosphorylation,tumorigenesis pathway,transcriptional regulation,DNA damage response,adipocyte differentiation and embryonic development.The target genes of hsa-miR-374b-5p mainly regulate gene expression,regulate the activity of sequence-specific DNA binding transcription factors,translation regulation,cell response to insulin,cell adhesion,cell cycle and other biological processes.The results of signal pathway enrichment are mainly distributed in TNF signaling pathway,RAS signaling pathway,human papillomavirus infection,transcriptional error regulation and various tumor signaling pathways.Conclusion The important regulatory role of long non-coding RNA HK2 in cells is closely related to multiple signaling pathways such as protein phosphorylation,tumorigenesis pathways,transcriptional regulation,DNA damage response,translation,and cell cycle.It mainly regulates physiological and pathological processes through the above-mentioned pathways.

关 键 词：长链非编码RNA-Hexokinase 2 信号通路 蛋白磷酸化 

分 类 号：Q343.1[生物学—遗传学] Q811.4