1998-2021年我国人感染H9N2亚型禽流感病毒的遗传演化规律  

Evolution of Human H9N2 Avian Influenza Virus in China from 1998 to 2021

作　　者：王艳文 王梦静 张虹 高鑫鑫 郭晶[1] 李旭勇[1] WANG YanWen;WANG MengJing;ZHANG Hong;GAO XinXin;GUO Jing;LI XuYong(College of Agronomy,Liaocheng University,Liaocheng 252000,Shandong)

机构地区：[1]聊城大学农学院,山东聊城252000

出　　处：《中国农业科学》2022年第20期4075-4090,共16页Scientia Agricultura Sinica

基　　金：山东省自然科学基金(ZR2021MC087);兽医生物技术国家重点实验室开放基金(SKLVBF201906,SKLVBF202005)。

摘　　要：【目的】通过分析1998—2021年间我国人感染H9N2亚型禽流感病例的发病时间、所在省份、年龄和性别等信息,明确H9N2亚型禽流感病毒的流行病学特征;通过分析人源H9N2亚型禽流感病毒的基因特征,阐明人源H9N2亚型禽流感病毒的遗传演化规律;为H9N2亚型禽流感病毒跨种间传播的预警和防控提供数据支撑。【方法】基于流感基因数据库、病例报道和文献资料,获得1998—2021年我国人感染H9N2亚型禽流感病毒的病例信息和毒株序列数据。从时间、空间、性别和年龄的分布对感染病例进行分析,明确人源H9N2亚型禽流感病毒感染的流行病学特征。通过DNASTAR中的MegAlign软件对人源H9N2病毒的各基因片段的核苷酸序列进行同源性分析,利用MEGA7.0软件构建系统进化树和分析病毒蛋白关键位点,揭示遗传演化趋势和病毒蛋白关键氨基酸位点的变异情况。通过GISAID网站下载2019—2021年间我国H9N2亚型禽流感病毒的核苷酸序列,利用mafft比对后在MEGA7.0中查看人源与禽源H9N2病毒关键氨基酸位点的突变差异,揭示当前人源和禽源H9N2病毒可能引起的风险。【结果】1998—2021年我国人感染H9N2亚型禽流感病毒病例共71例,从空间分布分析,病例分布于16个省市,其中91.55%的病例来自于南方12个省市;从时间分布分析,2013年以后,我国报道的感染病例呈增长趋势,2013—2021年累计感染病例数占总病例数的61.97%;从性别和年龄分布分析,男、女性别比为1:1.68,感染病例主要见于幼儿和少儿,占总病例的74.14%。对人源H9N2病毒进行基因组比对分析,发现这些病毒均属于欧亚分支,但是这些病毒各基因片段的核苷酸序列同源性差异较大,HA、NA、PB2、PB1、PA、NP、M和NS的同源性分别为75.3%—100%、80.1%—100%、78.7%—100%、82.5%—100%、72.6%—100%、74.1%—100%、65.5%—100%、82.0%—100%;22株具有完整基因片段的病毒分为8个基因�【Objective】 The epidemiological characteristics of H9N2 avian influenza virus were clarified by analyzing the onset time,province, age, gender and other information of human cases infected with H9N2 avian influenza from 1998 to 2021 in China. By analyzing the genetic characteristics of human H9N2 subtype avian influenza virus, the genetic evolution law of human H9N2subtype avian influenza virus was elucidated. This study could provide data support for the early warning and control of H9N2subtype avian influenza virus cross-species transmission. 【Method】 According to the case report, literature data and gene bank, the case information and strain sequence data of human infection with H9N2 subtype avian influenza virus in China from 1998 to 2021were obtained. The infection cases were analyzed from the time distribution, space distribution, gender and age distribution, and the epidemiological characteristics of human-derived H9N2 subtype avian influenza virus infection were found. The MegAlign software in DNASTAR were used to analyze Nucleotide sequence of the homology of various gene fragments of human H9N2 isolates, a phylogenetic tree was constructed by MEGA7.0 software, and the key sites of viral proteins were analyzed. The genetic evolution trend of viral proteins and the variation of key amino acid sites were clarified. The 2019-2021 H9N2 subtype avian influenza virus nucleotide sequence in China was downloaded from the GISAID website. After mafft alignment, the key amino acid site mutation differences between human and avian H9N2 viruses were examined in MEGA7.0, the potential risks brought by current human and avian H9N2 viruses were revealed. 【Result】 From1998 to 2021, a total of 71 cases of human infection with the H9N2 subtype avian influenza virus occurred in China. From the spatial distribution analysis, the cases were distributed in 16 provinces and cities, of which 91.55% of the cases were from 12 provinces and cities in the south. In terms of time, the number of reported infections has bee

关 键 词：禽流感病毒 H9N2亚型 流行病学 遗传进化 跨种间传播 

分 类 号：S852.65[农业科学—基础兽医学]