北京市昌平区腹泻患者弯曲菌感染现状及其分子特征分析  

Infection status and molecular characteristics of campylobacter isolated from diarrhea patients in Changping District of Beijing

作　　者：李东迅[1] 王维钧[1] 彭华[1] 银安琪 舒高林 牛桓彩[1] LI Dong-xun;WANG Wei-jun;PENG Hua;YIN An-qi;SHU Gao-lin;NIU Huan-cai(Department of Microbiology,Changping District Center for Disease Control and Prevention,Changping,Beijing 102200,China)

机构地区：[1]北京市昌平区疾病预防控制中心微生物检验科,北京102200

出　　处：《公共卫生与预防医学》2022年第6期123-127,共5页Journal of Public Health and Preventive Medicine

摘　　要：目的了解北京市昌平区腹泻患者弯曲菌感染现状及其分子流行特征,为该地区弯曲菌腹泻病的预防控制提供科学依据。方法收集2019年北京市昌平区某哨点医院腹泻患者粪便样本382份,进行弯曲菌、沙门菌、致泻大肠埃希菌、副溶血性弧菌检测,并对分离到的空肠弯曲菌进行脉冲场凝胶电泳分型(PFGE)分析。结果382份样本中,弯曲菌(58株,15.18%)检出率高于致泻大肠埃希菌(37株,9.69%)、沙门菌(24株,6.28%)和副溶血性弧菌(21株,5.50%),差异有统计学意义(χ^(2)=26.735,P=0.000)。季节分析显示:弯曲菌春季(14.71%)、夏季(18.33%)、秋季(15.00%)、冬季(10.00%)四季均有检出,但其差异无统计学意义(χ^(2)=2.197,P=0.533)。PFGE分析显示:30株空肠弯曲菌具有26种PFGE带型,相似系数为31.00%~100.00%,其PFGE带型具有多样性。结论弯曲菌检出率居于首位,四季检出,提示昌平区弯曲菌感染较严重,应引起重视,并制定相应的方针政策,防止由弯曲菌感染引起的疫情发生。Objective To understand the infection status and molecular characteristics of campylobacter,and to provide evidence for the prevention and control of campylobacter infection.Methods A total of 382 stool samples from diarrhea patients in a sentinel hospital in Changping District,Beijing,were collected in 2019.campylobacter,salmonella,diarrheaescherichia coli,and vibrio parahaemolyticus were detected,and the isolated campylobacter jejuni strains were analyzed by pulsed-field gel electrophoresis(PFGE).Results The positive detection rate of campylobacter(58 strains,15.18%)was higher than that of diarrhea escherichia coli(37 strains,9.69%),salmonella(24 strains,6.28%)and Vibrio parahaemolyticus(21 strains,5.50%),and the difference was statistically significant(χ^(2)=26.735,P=0.000).campylobacter strainswere found in spring(14.71%),summer(18.33%),autumn(15.00%)and winter(10.00%),but there was no significant difference(χ^(2)=2.197,P=0.533).PFGE analysis showed that 30 strains of campylobacter jejuni had 26 PFGE patterns,and the similarity coefficient was 31.00%-100.00%.Conclusion In the study,the detection rate of campylobacter is in the first place,and it is detected in four seasons,indicating that the infection of campylobacter is serious in Changping District of Beijing.It is necessary to pay more attention to the infection of campylobacter and formulate corresponding policies to prevent the outbreak caused by campylobacter infection.

关 键 词：腹泻患者 弯曲菌 空肠弯曲菌 结肠弯曲菌 脉冲场凝胶电泳 

分 类 号：R181[医药卫生—流行病学]