PIGF和PEG3在辅助生殖妊娠与自然妊娠胎盘中的差异表达及临床意义  

Differential expression and clinical significance of PIGF and PEG3 in placenta of assisted reproduction or natural pregnancy

作　　者：邹薇 蒋梦芬 马天仲 ZOU Wei;JIANG Meng-fen;MA Tian-zhong(Reproductive Medicine Center of the Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001)

机构地区：[1]广东医科大学附属医院生殖医学中心,湛江524001

出　　处：《生殖医学杂志》2022年第11期1561-1567,共7页Journal of Reproductive Medicine

基　　金：广东省自然科学基金(2022A1515010849);广东省医学科研基金项目(B2019007);湛江市科技发展专项资金竞争性分配项目(2019A01023)。

摘　　要：目的 分析辅助生殖技术(ART)与妊娠并发症及子代不良结局的相关性,探讨ART技术对胎盘生长因子(PIGF)和父系表达基因3(PEG3)的影响。方法 收集2021年9—12月在广东医科大学顺德妇女儿童医院产科分娩的产妇资料,根据妊娠方式分为ART组(经ART获得妊娠,n=113)和自然妊娠组(n=153)。比较两组产妇的基本资料、妊娠期并发症及子代不良结局。分别收集两组的胎盘组织各18例,标记为自然妊娠组(A1-A18)和ART组(B1-B18),HE染色观察两组胎盘组织的形态结构,qRT-PCR检测PIGF和PEG3的mRNA表达水平,Western blot及免疫组化检测PIGF和PEG3的蛋白表达水平及定位,统计分析两组间的差异。结果 与自然妊娠组相比,ART组的产妇年龄、体质量指数(BMI)、剖宫产比例显著上升,产次显著下降(P均<0.05),妊娠期高血压和妊娠期糖尿病(GDM)的发生率显著升高(P<0.05)。ART组的新生儿早产及低出生体重儿比率显著高于自然妊娠组(P<0.05)。qRT-PCR结果显示,ART组产妇胎盘组织中PIGF的mRNA表达水平显著低于自然妊娠组(P<0.05),而PEG3的mRNA表达水平在两组间无显著性差异(P>0.05)。Western blot结果显示,ART组胎盘组织中的PEG3蛋白灰度值显著低于自然妊娠组(P<0.05),而PIGF蛋白的灰度值有低于自然妊娠组的趋势,但差异尚无统计学意义(P>0.05)。HE染色结果显示,ART组和自然妊娠组胎盘光镜下结构大体正常;免疫组化结果显示,PEG3蛋白主要表达于胎盘合体滋养细胞的细胞膜中,PIGF蛋白主要表达于胎盘的合体滋养细胞的细胞膜和细胞质中。结论 通过ART助孕的产妇,孕期发生妊娠期高血压和GDM的几率增加,且新生儿不良结局发生风险增加;ART助孕影响胎盘组织中PIGF和PEG3 mRNA及蛋白的表达,但是不影响胎盘结构,ART助孕对胎盘的影响还需进一步研究。Objective:To analyze the correlation between assisted reproductive technology(ART)and pregnancy complications and adverse outcomes of offspring,and to investigate the effect of ART on placenta growth factor(PIGF)and patrilineal expression gene 3(PEG3).Methods:The data of women who delivered in the Department of Obstetrics,Shunde Women and Children’s Hospital of Guangdong Medical University from September to December 2021 were collected.The women divided into ART group(n=113)and natural pregnancy group(n=153)according to pregnancy type.The basic data,pregnancy complications and adverse outcomes of offspring were compared between the two groups.The placental tissues of 18 women in each group were collected and labeled as natural pregnancy group(A1-A18)and ART group(B1-B18).HE staining was used to observe the morphological structure of placental tissues of the two groups.The mRNA expression levels of PIGF and PEG3 were detected by qRT-PCR.Western blot and immunohistochemistry were used to detect the protein expression levels and localization of PIGF and PEG3,and the differences between the two groups were statistically analyzed.Results:Compared with the natural pregnancy group,the age,body mass index(BMI)and cesarean section proportion of women in ART group were significantly increased and the parity was significantly decreased(P<0.05),and the incidence of gestational hypertension and gestational diabetes(GDM)was significantly increased(P<0.05).The rates of premature birth and low birth weight infants in ART group were significantly higher than those in natural pregnancy group(P<0.05).The results of qRT-PCR showed that the mRNA expression level of PIGF in the placenta of ART group was significantly lower than that of natural pregnancy group(P<0.05),while the mRNA expression level of PEG3 had no significant difference between the two groups(P>0.05).Western blot results showed that the gray value of PEG3 protein in placenta of ART group was significantly lower than that of natural pregnancy group(P<0.05),while the g

关 键 词：辅助生殖技术 父系表达基因3(PEG3) 胎盘生长因子(PIGF) 妊娠并发症 

分 类 号：R711.6[医药卫生—妇产科学] R714.12[医药卫生—临床医学]