番茄果实灰霉病拮抗细菌菌株SG-11的鉴定及其抑菌作用  

Identification of Bacterial Strain SG-11 against Botrytis cinerea and Its Bacteriostasis

作　　者：崔杰 王翠翠 刘永光[1] 赵红 张德珍[1] 迟文娟 杨园园 Cui Jie;Wang Cuicui;Liu Yongguang;Zhao Hong;Zhang Dezhen;Chi Wenjuan;Yang Yuanyuan(Weifang University of Science and Technology/Shandong Facility Horticulture Bioengineering Research Center/Shandong Provincial University Laboratory for Protected Horticulture,Shouguang 262700,China;Natural Resources Bureau of Huangdao District,Qingdao City,Qingdao 266555,China)

机构地区：[1]潍坊科技学院/山东省设施园艺生物工程研究中心/山东省高校设施园艺实验室,山东寿光262700 [2]青岛市黄岛区自然资源局,山东青岛266555

出　　处：《山东农业科学》2022年第10期104-109,共6页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(32001854);山东省大学生创新训练项目(S202112843003);潍坊科技学院博士启动基金项目(2021KJBS04)。

摘　　要：为开发对番茄果实灰霉病有效的生防细菌,本试验对从山东寿光温室番茄根际土壤中分离的28株细菌进行了筛选,获得有显著拮抗作用的菌株SG-11,采用对峙培养法测定了其对番茄灰霉病菌菌丝生长和孢子萌发的影响,并采用16S rDNA和gyrB基因序列对菌株SG-11进行了分子生物学鉴定。结果表明,菌株SG-11对番茄灰霉病菌抑制率为79.81%,其上清滤液能有效抑制孢子萌发并导致菌丝畸形,且对10种常见植物病原菌有显著拮抗作用。经鉴定该菌株为贝莱斯芽孢杆菌(Bacillus velezensis),其不同稀释倍数的滤液能显著降低番茄果实灰霉病的发病面积,稀释10倍的防效仍高于80%,表明该菌株具有良好的生防潜力。In order to develop effective biocontrol bacteria against Botrytis cinerea,28 strains of bacteria isolated from tomato rhizosphere soil in Shouguang greenhouses of Shandong Province were screened,and the strain SG-11 with significant antagonistic effect on Botrytis cinerea was obtained.The method of confrontation culture was used for testing the antagonistic effect of SG-11 on hyphal growth and spore germination of Botrytis cinerea,and it was identified by 16 S rDNA and gyrB gene sequences.The results showed that the inhibition rate of SG-11 to Botrytis cinerea was 79.81%,and its supernatant could effectively inhibit spore germination and cause hyphal deformity,and had significant antagonistic effect against 10 common plant pathogens.The strain was identified as Bacillus velezensis.Its filtrate with different dilution times could significantly reduce the incidence of Botrytis cinerea on tomato fruits.The control effect of 10 times of dilution was still higher than 80%,which indicated that the strain had good biocontrol potential.

关 键 词：番茄灰霉病菌 贝莱斯芽孢杆菌 抑菌作用 防效 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]