鸭坦布苏病毒感染对occludin和claudin-1基因mRNA表达的影响  

Effect of Duck Tembusu Virus Infection on mRNA Expression of occludin and claudin-1 Genes

作　　者：逯璐 苏兴海 马世宝 张琳[4] Lu Lu;Su Xinghai;Ma Shibao;Zhang Lin(Emerging Economic Formats Research Institute of Shandong Management University,Jinan 250357,China;Zhaoyuan Center for Animal Disease Control and Prevention,Zhaoyuan 265400,China;Lingcheng District Animal Husbandry and Veterinary Service Center of Dezhou City,Dezhou 253599,China;Institute of Animal Science and Veterinary Medicine,Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Animal Disease Control and Animal Breeding,Jinan 250100,China)

机构地区：[1]山东管理学院新兴业态发展研究所,山东济南250357 [2]招远市动物疫病预防控制中心,山东招远265400 [3]德州市陵城区畜牧兽医服务中心,山东德州253599 [4]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100

出　　处：《山东农业科学》2022年第10期122-126,172,共6页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2019QC013)。

摘　　要：紧密连接蛋白不仅是维持上皮屏障功能的重要蛋白,更是多种病毒感染细胞的受体与辅助分子。为了明确紧密连接主要组成蛋白occludin和claudin-1在鸭坦布苏病毒(Duck Tembusu virus, DTMUV)入侵细胞中的作用,本试验利用荧光定量PCR方法对病毒感染的非洲绿猴肾(vero)细胞两基因mRNA的表达量进行动态检测。结果表明,occludin和claudin-1的表达量与病毒含量具有相关性,感染后48 h内随着病毒含量的增加,occludin和claudin-1的mRNA上调表达,当病毒拷贝数下降且细胞出现明显病变后,两基因变为下调表达。试验结果有助于明确occludin和claudin-1在介导DTMUV入侵细胞中的作用,为后续功能受体的筛选和抗病毒制剂的研发提供依据。Tight junction proteins are not only important for maintaining epithelial barrier function but also receptors and accessory molecules of many viruses infect cells. In order to define the roles of occludin and claudin-1, major components of tight junction proteins, in Duck Tembusu virus(DTMUV) invasion cells, the dynamic changes of mRNA expression of the two genes in vero cells were detected by fluorescence quantitative PCR(qPCR). The results indicated that the mRNA expression level of occludin and claudin-1 were correlated with the virus content. With the increase of virus content within 48 hours after infection, the mRNA expression of occludin and claudin-1 was up-regulated, and then downregulated with the cytopathic effect(CPE) emergence and virus copies decreasing. These findings would help clarify the role of occludin and claudin-1 in mediating DTMUV invading cells and provide references for identification of functional receptors and development of antiviral reagents for DTMUV.

关 键 词：鸭坦布苏病毒 OCCLUDIN CLAUDIN-1 定量检测 

分 类 号：S852.657[农业科学—基础兽医学]