尸蓝蝇虫卵的分子鉴定与遗传进化分析  

MOLECULAR IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF THE EGGS OF CYNOMYA MORTUORUM(LINNAEUS,1761)

作　　者：张学勇[1] 简莹娜 李志 马怡隽 王永顺[2] 刘佳[2] 张青 王威[2] 朵红[1] 郭志宏[1] 沈秀英[1] 付永[1] ZHANG Xue-yong;JIAN Ying-na;LI Zhi;MA Yi-juan;WANG Yong-shun;LIU Jia;ZHANG Qing;WANG Wei;DUO Hong;GUO Zhi-hong;SHEN Xiu-ying;FU Yong(Academy of Animal and Veterinary Sciences,Qinghai University,Xining 810016,Qinghai,China;Qinghai Province Institute for Endemic Diseases Prevention and Control,Xining 810016,Qinghai,China)

机构地区：[1]青海大学畜牧兽医科学院,西宁810016 [2]青海省地方病预防控制所,西宁810016

出　　处：《寄生虫与医学昆虫学报》2022年第3期163-168,共6页Acta Parasitologica et Medica Entomologica Sinica

基　　金：牧科院基本科研业务费自主选题项目(MKY-2019-10)。

摘　　要：为鉴定田鼠源蝇类虫卵,通过PCR分子生物学方法扩增该虫卵样本的COⅠ基因和28S rRNA基因序列,并进行了序列与系统发育分析。结果显示,克隆的COⅠ基因和28S rRNA基因序列分别与GenBank上的C.mortuorum核苷酸参考序列的同源性高达99.23%和99.56%以上,蝇类虫卵鉴定为尸蓝蝇Cynomya mortuorum;利用GenBank中其他蝇类的COⅠ和28S rRNA基因序列构建进化树,该蝇类虫卵与已鉴定的C.mortuorum聚在同一微小分支上,而与其他蝇类所属分支相距较远。本研究首次在分子水平上鉴定了青海田鼠源C.mortuorum,可为青藏高原地区C.mortuorum区系分布调查与分子溯源工作提供参考资料。The purpose of present study was to identify the fly eggs derived from voles.The COⅠand 28S rRNA gene sequences of the egg samples were amplified by PCR molecular method,and sequenced and phylogenetic analysis was then carried out,respectively.The results showed that the homogeneity of cloned COⅠand 28S rRNA gene sequences was as high as 99.23%and 99.56%to the nucleotide reference sequence of C.mortuorum in GenBank,the fly eggs were identified as Cynomya mortuorum by molecular identification.Meanwhile,the COⅠand 28S rRNA gene sequences were applied to construct phylogenetic trees along with the other fly species deposited in GenBank.And the fly egg was clustered in the same tiny branches with C.mortuorum identified in the two phylogenetic trees,which separated far away from the branches of other fly species.To our knowledge,it was the first time to identify C.mortuorum from the Qinghai vole at the molecular level,and to study its phylogenetic relationship with other flies.The present study will provide reference data for the fauna investigations and molecular trace of C.mortuorum in the Qinghai-Tibet Plateau.

关 键 词：尸蓝蝇 分子鉴定 遗传进化 田鼠 青海 

分 类 号：Q963[生物学—昆虫学]