谷子茎尖诱导愈伤的品种筛选及分化条件探究  

The Screening of Varieties for Callus Induction and Differentiation Conditions in Foxtail Millet

作　　者：祁东梅 史慎奎 王玉芳 王春芳[1] QI Dongmei;SHI Shenkui;WANG Yufang;WANG Chunfang(College of Biological and Food Science,Hebei Normal University for Nationalities,Chengde,Hebei 067000,China)

机构地区：[1]河北民族师范学院生物与食品科学学院,河北承德067000

出　　处：《作物研究》2022年第5期464-471,共8页Crop Research

基　　金：承德市科技局项目(202109A196);河北民族师范学院青年基金(QN2021002);河北省高等学校科学技术研究项目(QN2022188,ZD2022151)。

摘　　要：对易形成愈伤组织的不同谷子品种进行筛选,优化愈伤分化体系,为谷子功能基因的遗传转化奠定基础。本研究对31个宜在承德本地播种的谷子品种进行筛选,以茎尖为材料进行组织培养,综合分析筛选出发芽率高、愈伤组织诱导率高、染菌率低的4个品种分别是:红粘谷、9115、长农35、师院-1。后续研究以师院-1的茎尖为实验材料,探究谷子愈伤组织诱导及分化最适条件。结果显示,2,4-D浓度为1.0 mg/L时,愈伤诱导率最高;KT与1.0 mg/L 2,4-D搭配诱导愈伤的最适浓度为0.1 mg/L;ZT与1.0 mg/L 2,4-D搭配诱导愈伤的最适浓度为1.0 mg/L。选择胚性愈伤组织进行分化条件探究,结果表明,诱导愈伤分化的激素配比为1.0 mg/L 2,4-D+1.0 mg/L TDZ,生芽率为35%;2.0 mg/L 6-BA+0.6 mg/L NAA,生芽率为28%。本研究为谷子茎尖组织培养和遗传转化研究提供参考。Selecting the varieties suitable to form callus from different genotypes of millet,and optimizing the callus differentiation would provide foundation for the genetic transformation of functional genes in millet.In this study,31 millet varieties which were easy to be sown locally in Chengde were selected,and the shoot apex were used as tissue culture material,to obtain the varieties with high rate of germination and callus induction,low rate of bacterial infection.The result showed that among the 30 varieties,four kinds of varieties including Hongniangu,9115,Changnong 35 and Shiyuan-1 were eligible.The following study in exploring optimal conditions for callus induction and differentiation were using shoot apex of shiyuan-1 as experimental materials.The results showed that the optimal concentration of 2,4-D was 1.0 mg/L in callus formation.The optimal concentration of KT was 0.1 mg/L,ZT was 1.0 mg/L when they used together with 2,4-D.Embryogenic callus were selected to explore the conditions of differentiation.The results showed that the ratio of hormones to induce callus were 1.0 mg/L 2,4-D+1.0 mg/L TDZ,and the differentiation rate reached 35%.2.0 mg/L 6-BA+0.6 mg/L NAA,and the differentiation rate reached 28%.This study provided fundamental data for shoot apex tissue culture and genetic transformation of foxtail millet.

关 键 词：谷子 茎尖 愈伤诱导 品种筛选 分化 

分 类 号：S515.01[农业科学—作物学]