单细胞测序技术及其在畜禽遗传育种中的应用研究新进展  

New Progress on Single Cell Sequencing Technology and Its Application to Animal Genetics and Breeding

作　　者：彭巍[1] 贾可欣 张子敬[3] 刘贤 蔡翠翠[2] 李欣淼 雷初朝[2] 陈宏[2] 黄永震[2] PENG Wei;JIA Kexin;ZHANG Zijing;LIU Xian;CAI Cuicui;LI Xinmiao;LEI Chuzhao;CHEN Hong;HUANG Yongzhen(Qinghai Academy of Animal Science and Veterinary Medicine,Qinghai University,Qinghai Xi’ning 810016,China;College of Animal Science and Technology,Northwest Agriculture and Forestry University,Shaanxi Yangling 712100,China;Institute of Animal Husbandry and Veterinary Science,Henan Academy of Agricultural Sciences,Henan Zhengzhou 450002,China;Henan Provincial Animal Husbandry General Station,Henan Zhengzhou 450008,China)

机构地区：[1]青海省畜牧兽医科学院,青海大学,青海西宁810016 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]河南省农业科学院畜牧兽医研究所,河南郑州450002 [4]河南省畜牧总站,河南郑州450008

出　　处：《中国畜牧杂志》2022年第10期44-49,共6页Chinese Journal of Animal Science

基　　金：青海省科技计划(2021-ZJ-736);国家肉牛牦牛产业技术体系专项(CARS-37);河南省肉牛产业技术体系(S2013-08);围原市农业农村和社会发展领域科技项目(2019GKNS007);国家重点研发计划项目子课题(2018YFD0502304);河南省农业科学院科技创新创意项目(2020CX09);河南省农业科学院优秀青年科技基金(2020YQ36);西北农林科技大学2020年大学生创新创业训练计划项目(S202010712200、S202010712735、X202010712088)。

摘　　要：单细胞测序技术是指在单个细胞的水平上对细胞自身携带的遗传信息进行测序,包含对基因组、转录组、蛋白质组、表观遗传组的测序以及多组学联合测序等。目前,单细胞测序技术被广泛应用于人类疾病研究如肝脏疾病、心脏疾病、肿瘤等,其在畜禽遗传育种中也发挥了重大作用,利用单细胞测序技术可以对家畜家禽进行早期选种选育,缩短世代间隔,提高繁殖效率。本文对单细胞测序技术及其在畜禽遗传育种中的应用展开综述,以期为后续家畜家禽遗传育种的研究提供参考。Single-cell sequencing technology refers to the sequencing of genetic information carried by a cell itself at the level of a single cell, including genome sequencing, transcriptome sequencing, proteome sequencing, epigenome sequencing and multi-omics combined sequencing. At present, the technology is widely used in the study of human diseases such as liver disease, heart disease, cancer, etc., in livestock and poultry genetics and breeding also plays an important role. Single cell sequencing technology can be used for livestock and poultry early selection and breeding of animals, shorten generation interval and improve reproductive efficiency. In this paper, the single-cell sequencing technology and its application to livestock and poultry genetics and breeding were reviewed in order to provide some reference for the future research of livestock and poultry genetics and breeding.

关 键 词：单细胞测序 单细胞分离 家畜家禽育种 

分 类 号：S813.1[农业科学—畜牧学]