新疆维吾尔族人群16个X-STR基因座的遗传多态性  

Genetic Polymorphism of 16 X-STR Loci in Xinjiang Uygur Population

作　　者：袁春艳 夏若成 张素华 陈丽琴[1] 王亚丽[1] 屈轶龄 杨光远 董新宇 柴思雨 李成涛 陶瑞旸 YUAN Chun-yan;XIA Ruo-cheng;ZHANG Su-hua;CHEN Li-qin;WANG Ya-li;QU Yi-ling;YANGGuang-yuan;DONG Xin-yu;CHAI Si-yu;LI Cheng-tao;TAO Rui-yang(Department of Forensic Medicine,Inner Mongolia Medical University,Hohhot 010030,China;Shang-hai Key Laboratory of Forensic Medicine,Key Laboratory of Forensic Science,Ministry of Justice,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai 200063,China;School of Biology and Basic Medical Sciences,Soochow University,Suzhou 215123,Jiangsu Province,China;School of Forensic Medicine,Shanxi Medical University,Taiyuan 030001,China;School of Preclinical Medicine of Zunyi Medical University,Zunyi 563000,Guizhou Province,China)

机构地区：[1]内蒙古医科大学法医学教研室,内蒙古呼和浩特010030 [2]司法鉴定科学研究院上海市法医学重点实验室司法部司法鉴定重点实验室上海市司法鉴定专业技术服务平台,上海200063 [3]苏州大学基础医学与生物科学学院,江苏苏州215123 [4]山西医科大学法医学院,山西太原030001 [5]遵义医科大学基础医学院,贵州遵义563000

出　　处：《法医学杂志》2022年第4期500-506,共7页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(82072123);万人计划青年拔尖人才资助项目(WRQB2019);上海市法医学重点实验室资助项目(21DZ2270800);上海市司法鉴定专业技术服务平台资助项目(19DZ2292700);司法部司法鉴定重点实验室资助项目。

摘　　要：目的研究新疆维吾尔族人群16个X-STR基因座的遗传多态性和群体遗传学参数。方法应用Goldeneye®DNA身份鉴定系统17X对502例(女性251例,男性251例)新疆维吾尔族无关个体进行16个X-STR基因座的复合扩增,应用3130xl基因分析仪对扩增产物进行检测,并统计等位基因频率及群体遗传学参数,计算维吾尔族与其他8个人群间的遗传距离,并根据遗传距离进行多维尺度分析以及系统发育树的构建。结果在502例新疆维吾尔族无关个体中,所检测的16个X-STR基因座共观察到67个等位基因,等位基因频率为0.0013~0.5724,PIC为0.5688~0.8553,女性CDP为0.999999999999999,男性CDP为0.999999999743071,三联体累积平均非父排除率为0.999999997791859,二联体累积平均非父排除率为0.999998989000730。维吾尔族人群与哈萨克族人群遗传距离较近,与汉族人群遗传距离较远。结论该16个X-STR基因座在维吾尔族人群中具有高度的多态性和较好的鉴别能力,可为该人群的法医学个体识别、亲权鉴定和群体遗传学研究提供有力的补充。Objective To study the genetic polymorphism and population genetic parameters of 16 X-STR loci in Xinjiang Uygur population.Methods The Goldeneye®DNA identification system 17X was used to amplify 16 X-STR loci in 502 unrelated individuals(251 females and 251 males).The amplified products were detected by 3130xl genetic analyzer.Allele frequencies and population genetic parameters were analyzed statistically.The genetic distances between Uygur and other 8 populations were calculated.Multidimensional scaling and phylogenetic tree were constructed based on genetic distance.Results In the 16 X-STR loci,a total of 67 alleles were detected in 502 Xinjiang Uygur unrelated individuals.The allele frequencies ranged from 0.0013 to 0.5724.PIC ranged from 0.5688 to 0.8553.The cumulative discrimination power in females and males were 0.999999999999999 and 0.999999999743071,respectively.The cumulative mean paternity exclusion chance in trios and in duos were 0.999999997791859 and 0.999998989000730,respectively.The genetic distance between Uygur population and Kazakh population was closer,and the genetic distance between Uygur and Han population was farther.Conclusion The 16 X-STR loci are highly polymorphic and suitable for identification in Uygur population,which can provide a powerful supplement for the study of individual identification,paternity identification and population genetics.

关 键 词：法医遗传学 多态现象 遗传 X染色体 短串联重复序列 遗传距离 系统发育树 维吾尔族 新疆 

分 类 号：DF795.2[医药卫生—法医学]