无茎黄芪根的化学成分生物活性研究  

Bioactivity of the Chemical Constituents of Astragalus acaulis Roots

作　　者：陈颖志 张伟 陈浩 单华 杨艺玲 姜北 Chen Yingzhi;Zhang Wei;Chen Hao;Shan Hua;Yang Yiling;Jiang Bei(College of Pharmacy,Dali University,Dali,Yunnan 671000,China;Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学院,云南大理671000 [2]云南省滇西抗病原植物资源筛选研究重点实验室,云南大理671000

出　　处：《大理大学学报》2022年第10期1-4,共4页Journal of Dali University

基　　金：国家自然科学基金项目(31170313,81960637)。

摘　　要：目的:研究无茎黄芪根的化学成分的抗氧化与镇痛活性。方法:采用醋酸致扭体实验、小鼠热水甩尾实验和1,1-二苯基-2-苦基苯肼(DPPH)自由基清除实验对无茎黄芪根分离得到的样品进行镇痛、抗氧化活性测试。结果:在醋酸致扭体实验中,5-甲氧基落叶松脂醇-4-O-β-D-葡萄糖苷对小鼠扭体抑制率高达78.8%;在小鼠热水甩尾实验中,给予实验小鼠丁香脂素-4-O-β-D-葡萄糖苷60 min后,该组小鼠的平均痛阈值较给药前增加了1.84 s,显示该化合物具有显著的外周镇痛活性。在DPPH自由基清除实验中,木脂素类成分落叶松脂醇-4-O-β-D-葡萄糖苷、5-甲氧基落叶松脂醇-4-O-β-D-葡萄糖苷清除DPPH自由基的平均IC_(50)值分别为38.40、36.53μg/mL,显示较好的抗氧化活性。结论:无茎黄芪根中木脂素类成分具有较好的抗氧化和外周镇痛活性,该研究为深入认识黄芪属植物根的生物活性提供了实验依据。Objective:To investigate the antioxidant and analgesic activities of the chemical components of Astragalus acaulis roots.Methods:The analgesic and antioxidant activities of samples isolated from A. acaulis roots were tested by acetic acid-induced writhing test,hot water tail-flick test and 1,1-diphenyl-2-picrylhydrazyl(DPPH) free radical scavenging test. Results:The writhing inhibition rate of 5-methoxylarch-4-O-β-D-glucoside was 78.80% in acetic acid-induced writhing in mice,while the mean pain threshold increased by 1.84 seconds in the hot water tail-flick test after the mice were given syringaresinol-4-O-β-D-glucoside for 60 minutes compared with that before administration,indicating that the compound had significant peripheral analgesic activity.In DPPH free radical scavenging experiment,larch alcohol-4-O-β-D-glucoside and 5-methoxylarch-4-O-β-D-glucoside displayed good antioxidant activity with the average IC_(50) values of 38.40 μg/mL and 36.53 μg/mL,respectively. Conclusion:The lignans from the roots of A. acaulis have good antioxidant and peripheral analgesic activities. The study provides experimental basis for further understanding the bioactivity of the roots of Astragalus.

关 键 词：无茎黄芪 根 木脂素类成分 生物活性 

分 类 号：R965[医药卫生—药理学]