波纹龙虾对亚硝酸盐胁迫响应的转录组学分析  

Transcriptomics analysis of response of scalloped spiny lobster Panulirus homarus to nitrite stress

作　　者：梁妃爽 梁华芳[1] 卓宏标 孙榕泽 王潘妹 温崇庆[1] LIANG Feishuang;LIANG Huafang;ZHUO Hongbiao;SUN Rongze;WANG Panmei;WEN Chongqing(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088

出　　处：《大连海洋大学学报》2022年第5期809-816,共8页Journal of Dalian Ocean University

基　　金：广东省科技厅农业攻关项目(2013B020308009);广东海洋大学科研项目(C16394)。

摘　　要：为了探究波纹龙虾Panulirus homarus对亚硝酸盐的胁迫响应机理,采用转录组学测序分析方法,进行了正常养殖条件下波纹龙虾(体质量为39.00 g±5.31 g)肝胰腺和质量浓度为80 mg/L亚硝酸盐胁迫7 d的肝胰腺转录组比较研究。结果表明:转录组共筛选得到264个差异表达基因,其中,86个基因上调表达,178个基因下调表达;GO功能注释显示,被注释的差异表达基因主要与结合、催化和代谢等功能有关;KEGG通路富集分析显示,差异表达基因在黏着斑、白细胞跨内皮层迁移、胰岛素抵抗、紧密连接、淀粉与蔗糖代谢等通路中显著富集;随机挑选9个差异表达基因进行qRT-PCR验证,发现荧光定量结果与测序结果基本一致。研究表明,在亚硝酸盐胁迫下,通过GO和KEGG分析发现差异表达基因主要富集在能量代谢、生长和免疫等通路中,挖掘出的数据可为后续研究提供科学参考。In order to investigate the mechanism of the response of scalloped spiny lobster Panulirus homarus to nitrite stress,the transcriptomics were comparatively analyzed in hepatopancreas of scalloped spiny lobster with body weight of(39.00±5.31)g exposed to under normal conditions(NO_(2)^(-)-N<0.01 mg/L,control group)and to 80 mg/L nitrite stress prepared by NaNO_(2) for 7 d by a transcriptomic sequencing approach.The results showed that 264 differentially related genes were screened,with up-regulated 86 and down-regulated 178.GO functional annotation results showed that the annotated differential genes were primarily involved in functions including binding,catalysis and metabolism,and KEGG pathway enrichment analysis showed that the differentially expressed genes were significantly enriched in focal adhesion,leukocyte transendothelial migration,insulin resistance,tight junctions,and starch and sucrose metabolism pathways.Nine differential genes were randomly selected for qRT-PCR validation,and the fluorescence quantification results were found to be in general agreement with the sequencing results.The findings will provide substantial reference for the research of gene functions in energy metabolism,growth and immunity in scalloped spiny lobster under nitrite stress.

关 键 词：波纹龙虾 亚硝酸盐胁迫 转录组 差异表达基因 

分 类 号：S968.22[农业科学—水产养殖]