三叶木通提取物对糖尿病小鼠模型炎症反应和糖脂代谢的影响  

Effects of Akebia trifoliata extract on inflammatory response and glucose lipid metabolism in diabetic mice models

作　　者：杨金博 赵晨光 张林铃 卞国勇 包春玲[2] 包斌[1,3,4] YANG Jinbo;ZHAO Chenguang;ZHANG Linling;BIAN Guoyong;BAO Chunling;BAO Bin(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Sixth People’s Hospital affiliated with Shanghai Jiaotong University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquatic Product Processing and Preservation,Shanghai 201306,China;Institute of Marine Science,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海交通大学附属第六人民医院,上海201306 [3]上海水产品加工与贮藏工程技术研究中心,上海201306 [4]上海海洋大学海洋科学研究院,上海201306

出　　处：《食品与发酵工业》2022年第21期67-73,共7页Food and Fermentation Industries

基　　金：上海市自然科学基金项目(21ZR1427300);上海市创新行动计划项目(19440741200);国家自然科学基金项目(82173731)。

摘　　要：研究药食同源植物三叶木通提取物对Ⅱ型糖尿病小鼠模型炎症反应及相关糖尿病指标的影响。萃取法获得三叶木通乙酸乙酯提取物,高脂高糖饲料联合链脲佐菌素注射法构建糖尿病小鼠模型,设置对照组、空白组、阳性对照组和高、中、低剂量处理组,记录小鼠体重和一般药理情况,尾静脉取血法测定小鼠空腹血糖值。口服葡萄糖耐量试验法测定小鼠餐后血糖恢复能力;酶联免疫法测定小鼠血清的肿瘤坏死因子α(tumor necrosis factor,TNF-α)、白介素6(interleukin-6,IL-6)水平;苏木精-伊红染色法分析小鼠肝脏组织形态学;全自动生化分析仪测定小鼠血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high-density lipoprotein,HDL-C)、低密度脂蛋白胆固醇(low-density lipoprotein,LDL-C)含量。与空白组相比,三叶木通提取物处理组小鼠TNF-α、IL-6、TG、TC、LDL-C水平极显著降低(P<0.01),HDL-C水平极显著升高(P<0.01),小鼠肝脏炎症反应降低,血糖含量降低,体重增加。三叶木通提取物可以调节Ⅱ型糖尿病小鼠模型的代谢紊乱,降低炎症反应,改善糖尿病症状。The purpose of this study is to explore the effect of Akebia trifoliata extract,a plant with the same medicine and food,on inflammatory response and related diabetes indicators in a mouse model of typeⅡdiabetes.The Akebia trifoliata was extracted,and high-fat and high-sugar feed combined with streptozotocin injection were used to set up diabetic mouse models.The control group,blank group,positive control group,high,medium,and low dose treatment groups were used to record the weight of mice and general pharmacological conditions.The tail vein blood collection method was used to determine the fasting blood glucose level of mice.The recovery ability of blood glucose after a meal in mice by oral glucose tolerance test and the serum levels of tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)in mice were detected.The automatic biochemical analyzer was used to determine the content of triglyceride(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C)in mouse serum.The results showed that compared with the blank group,the levels of TNF-α,IL-6,TG,TC and LDL-C in mice treated with Akebia trifoliata extract were significantly decreased(P<0.01).While the level of HDL-C was significantly increased(P<0.01).The liver inflammation in mice and blood sugar levels were reduced,accompanied by the increase in body weight.Akebia trifoliata extract can regulate the metabolic disorder of the typeⅡdiabetic mouse model,reduce inflammation,enhance immunity,and improve the symptoms of diabetes.

关 键 词：三叶木通 提取物 糖尿病 动物模型 炎症反应 

分 类 号：R587.1[医药卫生—内分泌] TS201.4[医药卫生—内科学]