茯苓多糖对2型糖尿病大鼠丝裂原激活的蛋白激酶通路及胰岛素抵抗的影响  

Effect of pachyman on mitogen-activated protein kinase pathway and insulin resistance in type 2 diabetic rats

作　　者：李乔[1] 张博[1] LI Qiao;ZHANG Bo(South District of Guang'anmen Hospital of China Academy of Chinese Medical Sciences,Beijing 102618,China)

机构地区：[1]中国中医科学院广安门医院南区内分泌科,北京102618

出　　处：《安徽医药》2022年第12期2379-2382,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的探究茯苓多糖(PAC)对2型糖尿病(T2DM)大鼠丝裂原激活的蛋白激酶(MAPK)通路及胰岛素抵抗(IR)的影响。方法2019年3—10月,采用高脂高糖喂养联合腹腔注射链脲佐菌素(STZ)建立T2DM大鼠模型,采用随机数字表法分为模型(T2DM)组、二甲双胍(MET)组(0.65 g·kg^(−1)·d^(−1))和PAC低(PAC-L,0.05 g·kg^(−1)·d^(−1))、中(PAC-M,0.10 g·kg^(−1)·d^(−1))、高剂量(PAC-H,0.20 g·kg^(−1)·d^(−1))组,每组12只。另取12只正常非T2DM大鼠为正常组(NC组),干预8周后测定空腹血糖、空腹血清胰岛素(FINS)、三酰甘油及肝组织均浆丙二醛、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性,采用蛋白质印迹法检测肝脏组织磷酸化p38丝裂原激活的蛋白激酶(p-p38MAPK)、磷酸化c-Jun氨基末端激酶(p-JNK)蛋白表达水平。结果与NC组比较,T2DM组大鼠血清空腹血糖、FINS、三酰甘油含量、胰岛素抵抗指数(HOMA-IRI)(62.56±5.11比5.81±1.05)、肝脏组织丙二醛含量、p-p38MAPK(1.35±0.08比0.16±0.03)和p-JNK(1.29±0.08比0.17±0.02)蛋白水平均显著升高(P<0.05),胰岛素敏感指数(ISI)[(0.86±0.05)×10^(−3)比(7.69±0.14)×10^(−3)]、肝脏组织GSH-Px、SOD活性均显著降低(P<0.05);与T2DM组比较,PAC-L组、PAC-M组、PAC-H组大鼠血清空腹血糖、FINS、三酰甘油含量、HOMA-IRI(49.47±4.56、31.14±3.03、26.59±2.16比62.56±5.11)、肝脏组织丙二醛含量、p-p38MAPK(1.04±0.06、0.89±0.03、0.39±0.02比1.35±0.08)和p-JNK(0.85±0.02、0.72±0.05、0.31±0.01比1.29±0.08)蛋白水平均显著降低(P<0.05),ISI[(1.51±0.11)×10^(−3)、(2.14±0.12)×10^(−3)、(3.73±0.15)×10^(−3)比(0.86±0.05)×10^(−3)]、肝脏组织GSH-Px、SOD活性均显著升高(P<0.05),呈剂量依赖性(P<0.05)。PAC-H组均优于MET组(P<0.05)。结论PAC可改善T2DM大鼠胰岛素抵抗,该作用可能与其抑制MAPK通路减轻氧化应激有关。Objective To investigate the effect of pachyman(PAC)on mitogen activated protein kinase(MAPK)pathway and insulin resistance(IR)in type 2 diabetic rats.Methods From March 2019 to October 2019,the T2DM rat model was established by high fat and high sugar feeding combined with intraperitoneal injection of streptozotocin(STZ).The rats were randomly divided into model(T2DM)group,metformin(MET)group(0.65g·kg^(−1)·d^(−1))and PAC low(PAC-L,0.05 g·kg^(−1)·d^(−1)),medium(PAC-M,0.10 g·kg^(−1)·d^(−1))and high(PAC-H,0.20 g·kg^(−1)·d^(−1))dose group,with 12 rats in each group.Another 12 non-T2DM rats were taken as normal group(NC group),After 8 weeks of intervention,the levels of fasting blood-glucose,fasting serum insulin(FINS),triglyceride,the activities of glu⁃tathione peroxidase(GSH PX),superoxide dismutase(SOD)and malondialdehyde were determined,the protein expression levels of phosphorylated p38 mitogen activated protein kinase(p-p38MAPK)and phosphorylated c-Jun amino terminal kinase(p-JNK)in liver tissue were detected by Western blot.Results Compared with NC group,the levels of serum fasting blood-glucose,FINS,triglyceride,HOMA-IRI(62.56±5.11 vs.5.81±1.05),malondialdehyde content of liver tissue,p-p38 MAPK(1.35±0.08 vs.0.16±0.03)and p-JNK(1.29±0.08 vs.0.17±0.02)protein in T2DM group increased significantly(P<0.05),while ISI[(0.86±0.05)×10^(−3)vs.(7.69±0.14)×10^(−3)],GSH-Px of liver tissue and SOD activity decreased significantly(P<0.05);compared with T2DM group,the levels of serum FBG,FINS,triglyceride,HOMA-IRI(49.47±4.56,31.14±3.03,26.59±2.16 vs.62.56±5.11),malondialdehyde content of liver tissue,p-p38 MAPK(1.04±0.06,0.89±0.03,0.39±0.02 vs.1.35±0.08)and p-JNK(0.85±0.02,0.72±0.05,0.31±0.01 vs.1.29±0.08)in PAC-L group,PAC-M group and PAC-H group decreased significantly(P<0.05),while ISI[(1.51±0.11)×10^(−3),(2.14±0.12)×10^(−3),(3.73±0.15)×10^(−3)vs.(0.86±0.05)×10^(−3)],GSH-Px of liver tissue and SOD activity increased significantly(P<0.05),which was dose-

关 键 词：糖尿病 2型 茯苓属 多糖类 胰岛素抵抗 丝裂原激活的蛋白激酶 大鼠 Sprague-Dawley 

分 类 号：R285.5[医药卫生—中药学]