三七总皂苷对脑梗死大鼠神经细胞Wnt/β-连环素信号通路的影响  

Effects of panax notoginseng saponins on neuron Wnt/β-catenin signaling pathway in rats with cerebral infarction

作　　者：芦君 LU Jun(The First Department of Neurology,Central Hospital of Xinwen Mining Group Co.,Ltd,Tai'an,Shandong 271200,China)

机构地区：[1]新汶矿业集团有限责任公司中心医院神经内一科,山东泰安271200

出　　处：《安徽医药》2022年第12期2395-2398,I0003,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的 探究三七总皂苷对脑梗死大鼠神经细胞Wnt/β-连环素(β-catenin)信号通路的影响。方法 该研究起止时间为2018年1月至2019年3月。60只雄性SD大鼠采用随机数字表法分为空白对照组、模型组、低剂量三七总皂苷组(75 mg/kg)和高剂量三七总皂苷组(150 mg/kg),各15只。将模型组、低剂量三七总皂苷组和高剂量三七总皂苷组大鼠制成脑梗死模型;低剂量、高剂量三七总皂苷组均腹腔注射三七总皂苷,空白对照组和模型组注射等量生理盐水,每日1次,注射3 d。使用改良神经功能缺损评分(mNSS)评价大鼠神经功能;使用Z-Longa评分评价大鼠姿势反射情况;检测各组大鼠脑梗死面积及脑含水率;使用蛋白质印迹法检测Wnt/β-catenin信号通路蛋白及自噬相关蛋白表达情况。结果 与空白对照组相比,模型组大鼠mNSS评分、大鼠姿势反射评分、脑梗死率、脑含水率、微管相关蛋白轻链3-Ⅱ(LC3-Ⅱ)蛋白表达水平升高(P<0.05),自噬蛋白P62表达、Wnt信号通路配体蛋白Wnt3a蛋白表达(0.25±0.09比1.68±0.25)和β-catenin蛋白表达(0.40±0.10比1.55±0.20)降低(P<0.05)。与模型组相比,低、高剂量三七总皂苷组大鼠mNSS评分、大鼠姿势反射评分、脑梗死率、脑含水率降低,LC3-Ⅱ蛋白表达降低(P<0.05),P62蛋白、Wnt3a蛋白(0.79±0.15,1.26±0.25比0.25±0.09)和β-catenin蛋白表达(1.23±0.25,0.90±0.15比0.40±0.10)升高(P<0.05)。与低剂量三七总皂苷组相比,高剂量三七总皂苷组大鼠mNSS评分、大鼠姿势反射评分、脑梗死率、脑含水率降低,LC3-Ⅱ蛋白表达降低(P<0.05),P62蛋白、Wnt3a蛋白(1.26±0.25比0.79±0.15)和β-catenin蛋白表达(1.23±0.25比0.90±0.15)升高(P<0.05)。结论 三七总皂可以通过促进Wnt/β-catenin信号通路蛋白表达激活Wnt/β-catenin信号通路,发挥调节大鼠神经元细胞的自噬,改善脑梗死大鼠神经损伤的作用。Objective To explore the effects of panax no to ginseng saponins(PNS) on neuron Wnt/β-catenin signaling pathway in rats with cerebral infarction.Methods The study period was from January 2018 to March 2019.Sixty male SD rats were divided into blank control group,model group,low-dose PNS group(75 mg/kg) and high-dose PNS group(150 mg/kg) using random number table method,with 15 cases in each group.Rats in model group,low-dose PNS group and high-dose PNS group were made into cerebral infarction models.The administration groups were intraperitoneally injected with PNS,while blank control group and model group were injected with the same volume of normal saline,once/d for 3 d.The nerve function of rats was evaluated by modified neurological severity score points(mNSS).The posture reflex was evaluated by Z-Longa scores.The cerebral infarct area and cerebral water rate in each group were detected.The expressions of Wnt/β-catenin signaling pathway proteins and autophagy-related proteins were detected by Western blotting.Results Compared with blank control group,mNSS score of posture reflex,cerebral infarction rate,brain water rate,expression level of microtubule-associated protein 1 light chain 3(LC3-Ⅱ) protein were increased in model group(P<0.05),and expression levels of autophagy protein P62,Wnt signaling pathway ligand Wnt3a protein(0.25±0.09 vs.1.68±0.25) and β-catenin protein(0.40±0.10 vs.1.55±0.20)were decreased(P<0.05).Compared with model group,mNSS,score of posture reflex,cerebral infarction rate,brain water rate and expression of LC3-Ⅱ protein were decreased in low-dose and high-dose PNS group(P<0.05),and expressions of P62 protein,Wnt3a protein(0.79±0.15),(1.26±0.25 vs.0.25±0.09) and β-catenin protein(1.23±0.25,0.90±0.15 vs.0.40±0.10) were increased(P<0.05).Compared with low-dose PNS group,mNSS,score of posture reflex,cerebral infarction rate and brain water rate were decreased in high-dose PNS group,expression of LC3-Ⅱ protein was decreased(P<0.05),and expressions of P62 protein,Wnt3a pro

关 键 词：脑梗死 人参皂甙类 三七 Β连环素 Wnt信号通路 神经细胞 自噬 大鼠 SPRAGUE-DAWLEY 

分 类 号：R285.5[医药卫生—中药学]