肋果沙棘和西藏沙棘转录因子bHLH94基因对海拔适应性分化的研究  

Study on Adaptive Differentiation of Transcription Factor bHLH94 Gene to Altitude in Hippophae neurocarpa and H.tibetana

作　　者：钱婷 赵凡 张玉洁 李雪丽 孙坤[1] 张辉[1] QIAN Ting;ZHAO Fan;ZHANG Yujie;LI Xueli;SUN Kun;ZHANG Hui(College of Life Science,Northwest Normal University,Lanzhou 730070)

机构地区：[1]西北师范大学生命科学学院,兰州730070

出　　处：《植物研究》2022年第6期976-985,共10页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(31660060)。

摘　　要：bHLH转录因子是植物体内的第二大类转录因子,在植物的生长发育、生理代谢及逆境应答过程中起着重要的作用。以肋果沙棘(Hippophae neurocarpa)和西藏沙棘(H. tibetana)为研究材料,通过转录组测序,筛选出受正选择作用的转录因子bHLH94基因,基于HnbHLH94、HtbHLH94基因序列和基因表达量分析,研究肋果沙棘和西藏沙棘bHLH94基因对海拔的响应机制。HnbHLH94和HtbHLH94基因分别编码338和335个氨基酸,Sanger法测序验证序列的正确性及二者DNA结合结构域之外的10个非同义突变位点,推测与该基因的适应性进化有关;qRT-PCR验证HnbHLH94基因表达量随海拔的升高而减小,HtbHLH94基因表达量随海拔的升高而增大,提示二者可能在干旱、冷冻及辐射等方面提供了对海拔适应的分子基础。综上,HnbHLH94和HtbHLH94基因在序列结构和表达量两个方面来响应海拔升高的生境条件。The bHLH proteins are the second large family of transcription factors,which play important roles in plant growth development,physiological metabolism and stress responses.In this study,Hippophae neurocarpa and H.tibetana were used as materials,the positive selection of the transcription factor bHLH94 gene was screened out using transcriptome sequencing.Based on the analysis of gene sequence and expression of HnbHLH94 and HtbHLH94,the response mechanism of bHLH94 gene in H.neurocarpa and H.tibetana adapted to altitude was studied.The HnbHLH94 and HtbHLH94 gene encoded 338 and 335 amino acids respectively.Sanger sequencing method verified the correctness of sequences,however there were 10 non-synonymous mutation sites outside the DNA binding domain of the two genes,which was speculated to be related to the adaptive evolution of the gene.As the altitude increased,the HnbHLH94 gene expression decreased,while the HtbHLH94 gene expression increased based on qRT-PCR technology.It suggested that gene might provide a molecular basis for adaptation to altitude,drought,freezing and radiation respectively,and the sequence structure and expression of HnbHLH94 and HtbHLH94 genes responded to the habitat conditions of elevation.

关 键 词：bHLH94转录因子 肋果沙棘 西藏沙棘 海拔 适应性分化 

分 类 号：S793.6[农业科学—林木遗传育种]