刚毛柽柳ThGRF2基因的克隆和渗透胁迫应答分析  

Cloning and Osmotic Stress Response Analysis of ThGRF2 from Tamarix hispida

作　　者：吴静[1] 王媛媛[1] 王丹妮 刘百超 刘中原[1] WU Jing;WANG Yuanyuan;WANG Danni;LIU Baichao;LIU Zhongyuan(Northeast Forestry University,State Key Laboratory of Tree Genetics and Breeding,Harbin 150040)

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2022年第6期1044-1051,共8页Bulletin of Botanical Research

基　　金：中央高校基本科研业务费专项资金资助项目(2572020AW09);黑龙江头雁创新团队计划(林木遗传育种创新研究团队)。

摘　　要：14-3-3蛋白通常也称为通用调节因子(general regulatory factors,GRF),是一类丝氨酸和苏氨酸磷酸化结合蛋白,通过与其他转录因子或信号蛋白相互作用参与调节细胞内基础代谢、信号传导、参与植物生长发育以及环境胁迫应答等一系列生理过程。本研究从刚毛柽柳干旱转录组中克隆获得一条干旱胁迫差异表达的ThGRF2基因。ThGRF2基因CDS片段全长为786 bp,编码261个氨基酸。相对分子质量为29.40 kDa,理论等电点(pI)为4.76。将ThGRF2基因构建到pROK2过表达载体上,瞬时转化刚毛柽柳,渗透胁迫前后生理指标结果显示,渗透胁迫后ThGRF2过表达提高了转基因柽柳的叶绿素含量、SOD和POD活性,降低了丙二醛(MDA)含量、电导率(EL)和失水率,表明ThGRF2基因在刚毛柽柳渗透胁迫应答中起重要作用。为进一步探究刚毛柽柳ThGRF2基因的非生物胁迫耐受性功能奠定基础。14-3-3 protein,also called general regulatory factors(GRF),is a type of serine and threoninephosphorylation binding protein,which participates in the regulation of series of physiological processes,suchas intracellular basis,signal transduction,plant growth and development,environmental stress response,byinteracting with other transcription factors or signaling proteins. In this study,the ThGRF2 gene that wasdifferentially expressed under drought stress and cloned from the drought transcriptome of Tamarix hispida. TheCDS fragment of ThGRF2 gene was 786 bp,encoded 261 amino acids. The relative molecular weight was 29.40k Da,and the theoretical isoelectric point was 4.76. The overexpression vector p ROKII-ThGRF2 wasconstructed,and transformed into T. hispida. The physiological index results showed that ThGRF2 overexpressionincreased the chlorophyll content,SOD and POD activities,and reduced malondialdehyde content(MDA),electrical conductivity(EL)and water loss rate under osmotic stress,indicated that ThGRF2 gene played animportant role in the response to osmotic stress of T.hispida. It laid a foundation for further exploration of theabiotic stress tolerance function of ThGRF2 gene.

关 键 词：刚毛柽柳 14-3-3蛋白 渗透胁迫 生理指标 

分 类 号：Q943.2[生物学—植物学]