铜绿微囊藻对蒙古裸腹溞生殖及抗氧化酶的影响  

Effects of Microcystis aeruginosa on Reproduction and Activities of Antioxidant Enzymes of Water Fleas Moina mongolica

作　　者：邢浩 陈桃英[2] 段元亮 郜晓峰 刘炜 钟逸云 张建琳 常佳楠 孙彬 何培民 XING Hao;CHEN Taoying;DUAN Yuanliang;GAO Xiaofeng;LIU Wei;ZHONG Yiyun;ZHANG Jianlin;CHANG Jianan;SUN Bin;HE Peimin(Shanghai University Engineering Research Center for Water Environment Ecology,School of Marine Ecology and Environment,Shanghai Ocean University,Shanghai 201306,China;Aquatic Ecology Laboratory,School of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学海洋生态与环境学院,水域环境生态上海高校工程研究中心,上海201306 [2]上海海洋大学水产与生命学院,水域生态学实验室,上海201306

出　　处：《水产科学》2022年第6期999-1006,共8页Fisheries Science

基　　金：国家科技支撑计划项目(2012BAC07B03);上海市海洋局科研项目(沪海科2015-02).

摘　　要：随着水体富营养化和全球变暖,微囊藻水华在低盐度封闭海域也时常发生。为探讨低盐度封闭海域中的铜绿微囊藻的毒性对盐水枝角类动物生殖、抗氧化酶的重要影响,在室内条件下培养耐盐为8的有毒铜绿微囊藻,分别开展不同藻密度的有毒铜绿微囊藻[4个有毒铜绿微囊藻处理组、1个对照组(小球藻)、1个禁食组]、不同组合的混合饵料(设置4个处理组和1个对照组,藻密度为1×10^(5)个/mL)对蒙古裸腹溞生长繁殖的影响,以及不同食物条件对蒙古裸腹溞体内超氧化物歧化酶和过氧化氢酶活性的影响。结果显示:在不同的藻密度条件下,随着有毒铜绿微囊藻藻密度增大,蒙古裸腹溞寿命显著减短,并且在有毒铜绿微囊藻处理组中均无繁殖现象;在混合饵料(小球藻+有毒铜绿微囊藻)条件下,有毒铜绿微囊藻占比越大,蒙古裸腹溞的净生殖率越低,世代时间越短;在96 h的酶活性测试中,与小球藻组相比,暴露在有毒铜绿微囊藻条件下的蒙古裸腹溞的超氧化物歧化酶和过氧化氢酶活性随时间显著下降。研究表明,有毒铜绿微囊藻显著抑制蒙古裸腹溞的种群生长。在2种食物条件下,添加小球藻可以缓解铜绿微囊藻对蒙古裸腹溞的毒害作用,同时铜绿微囊藻的毒性也显著影响蒙古裸腹溞的超氧化物歧化酶和过氧化氢酶活性。With the eutrophication of water bodies and global warming,microcystis blooms often occur in low-salinity enclosed sea areas.In order to explore the important effects of the toxicity of Microcystis aeruginosa in low-salinity enclosed seas on the reproduction and antioxidant enzymes of saltwater cladocerans,the toxic M.aeruginosa with a salt tolerance of 8 was cultivated under indoor conditions at different development stages.Density of toxic M.aeruginosa[4 toxic M.aeruginosa treatment groups,1 control group(Chlorella sp.),and 1 fasting group],mixed foods of different combinations(set 4 groups and 1 control group,with an alga density of 1×10^(5) ind./mL)on the growth and reproduction of water fleas Moina mongolica,and the effects of different food conditions on the activities of superoxide dismutase(SOD)and catalase(CAT)in M.mongolica.The results showed that under different alga density conditions,M.mongolica had significant reduce in lifespan as the density of toxic M.aeruginosa increased,and there was no reproduction in the toxic M.aeruginosa treatment group.Under the condition of mixed food(Chlorella sp.+toxic M.aeruginosa),the greater the proportion of toxic M.aeruginosa,the lower the net reproductive rate of M.mongolica,the shorter the generation time;in the 96-hour enzyme activity test,compared with the Chlorella sp.group,the superoxide dismutase and catalase activities of M.mongolica exposed to the toxic M.aeruginosa was significantly decreased over time.Therefore,it was found that the toxic M.aeruginosa significantly inhibited the life history characteristics of M.mongolica.Under the two food conditions,the addition of Chlorella sp.can alleviate the toxic effect of M.aeruginosa on M.mongolica.At the same time,the toxicity of M.aeruginosa also significantly affected the superoxide dismutase and catalase activities of M.mongolica.

关 键 词：蒙古裸腹溞 铜绿微囊藻 内禀增长率 超氧化物歧化酶 过氧化氢酶 

分 类 号：S917.4[农业科学—水产科学]